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PMID: 18332221 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Myoblasts and macrophages share molecular components that contribute to cell-cell fusion.

The Journal of cell biology ·Vol. 180 ·No. 5 ·2008-03-10 ·Pages 1005-19

Pajcini KV, Pomerantz JH, Alkan O, Doyonnas R, Blau HM

Abstract

Cell-cell fusion is critical to the normal development of certain tissues, yet the nature and degree of conservation of the underlying molecular components remains largely unknown. Here we show that the two guanine-nucleotide exchange factors Brag2 and Dock180 have evolutionarily conserved functions in the fusion of mammalian myoblasts. Their effects on muscle cell formation are distinct and are a result of the activation of the GTPases ARF6 and Rac, respectively. Inhibition of ARF6 activity results in a lack of physical association between paxillin and beta(1)-integrin, and disruption of paxillin transport to sites of focal adhesion. We show that fusion machinery is conserved among distinct cell types because Dock180 deficiency prevented fusion of macrophages and the formation of multinucleated giant cells. Our results are the first to demonstrate a role for a single protein in the fusion of two different cell types, and provide novel mechanistic insight into the function of GEFs in the morphological maturation of multinucleated cells.

MeSH Terms
ADP-Ribosylation Factor 6 ADP-Ribosylation Factors/genetics,metabolism Animals Cell Adhesion/physiology Cell Communication/physiology Cell Fusion Cell Line Focal Adhesions/metabolism,ultrastructure Giant Cells/metabolism,ultrastructure Guanine Nucleotide Exchange Factors/genetics,metabolism Integrin beta1/metabolism Macrophages/metabolism,ultrastructure Mice Myoblasts/metabolism,ultrastructure Neuropeptides/genetics,metabolism Paxillin/metabolism RNA, Small Interfering rac GTP-Binding Proteins/genetics,metabolism rac1 GTP-Binding Protein
Chemicals
ADP-Ribosylation Factor 6 DOCK180 protein, rat Dock180 protein, mouse Guanine Nucleotide Exchange Factors IQSEC1 protein, human Integrin beta1 Neuropeptides Paxillin RNA, Small Interfering Rac1 protein, mouse ADP-Ribosylation Factors ARF6 protein, human Arf6 protein, mouse Arf6 protein, rat rac GTP-Binding Proteins rac1 GTP-Binding Protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Pajcini Kostandin V
Department of Microbiology & Immunology, Baxter Laboratory in Genetic Pharmacology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Pomerantz Jason H
Alkan Ozan
Doyonnas Regis
Blau Helen M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
Published
2008-03-10
Pages
1005-19
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2265408
Subset
IM
Grants
NIA NIH HHS · R37 AG009521 · United States
NIA NIH HHS · AG024987 · United States
NIBIB NIH HHS · R01 EB005011 · United States
NIA NIH HHS · AG020961 · United States
NIA NIH HHS · AG009521 · United States
NIAID NIH HHS · T32 AI007328 · United States
ACF HHS · AF051678 · United States
NIA NIH HHS · R01 AG020961 · United States
NIA NIH HHS · R01 AG009521 · United States
NIBIB NIH HHS · EB005011 · United States
NICHD NIH HHS · R01 HD018179 · United States
NICHD NIH HHS · HD018179 · United States
NICHD NIH HHS · T32 HD007249 · United States
NIA NIH HHS · R01 AG024987 · United States
NICHD NIH HHS · 5T32 HD007249 · United States
NIAID NIH HHS · 5T32 AI07328 · United States
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