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PMID: 1833629 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a candidate bcl-1 gene.

Molecular and cellular biology ·Vol. 11 ·No. 10 ·1991-10-00 ·Pages 4846-53

Withers DA, Harvey RC, Faust JB, Melnyk O, Carey K, Meeker TC

Abstract

The t(11;14)(q13;q32) translocation has been associated with human B-lymphocytic malignancy. Several examples of this translocation have been cloned, documenting that this abnormality joins the immunoglobulin heavy-chain gene to the bcl-1 locus on chromosome 11. However, the identification of the bcl-1 gene, a putative dominant oncogene, has been elusive. In this work, we have isolated genomic clones covering 120 kb of the bcl-1 locus. Probes from the region of an HpaII-tiny-fragment island identified a candidate bcl-1 gene. cDNAs representing the bcl-1 mRNA were cloned from three cell lines, two with the translocation. The deduced amino acid sequence from these clones showed bcl-1 to be a member of the cyclin gene family. In addition, our analysis of expression of bcl-1 in an extensive panel of human cell lines showed it to be widely expressed except in lymphoid or myeloid lineages. This observation may provide a molecular basis for distinct modes of cell cycle control in different mammalian tissues. Activation of the bcl-1 gene may be oncogenic by directly altering progression through the cell cycle.

Related Genes
bcl-1
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular Cyclin D1 Cyclins/genetics Gene Expression/physiology Genes, Dominant/genetics Humans Molecular Sequence Data Multigene Family/genetics Oncogenes/genetics Proto-Oncogene Proteins/genetics Sequence Alignment Translocation, Genetic/genetics Tumor Cells, Cultured
Chemicals
Cyclins Proto-Oncogene Proteins Cyclin D1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Withers D A
Department of Medicine, University of California, San Francisco.
Harvey R C
Faust J B
Melnyk O
Carey K
Meeker T C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.10.4846-4853.1991
Published
1991-10-00
Pages
4846-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361453
Subset
IM
Grants
NCI NIH HHS · CA01102 · United States
Databases
GENBANK
M73554
External Links
PubMed source DOI: 10.1128/mcb.11.10.4846-4853.1991 PMC Full Text View journal details
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