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PMID: 18346202 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Immunoproteasome responds to injury in the retina and brain.

Journal of neurochemistry ·Vol. 106 ·No. 1 ·2008-07-00 ·Pages 158-69

Ferrington DA, Hussong SA, Roehrich H, Kapphahn RJ, Kavanaugh SM, Heuss ND, Gregerson DS

Abstract

It is well known that immunoproteasome generates peptides for MHC Class I occupancy and recognition by cytotoxic T lymphocytes (CTL). The present study focused on evidence for alternative roles for immunoproteasome. Retina and brain were analyzed for expression of immunoproteasome subunits using immunohistochemistry and western blotting under normal conditions and after injury/stress induced by CTL attack on glia (brain) or neurons (retina). Normal retina expressed substantial levels of immunoproteasome in glia, neurons, and retinal pigment epithelium. The basal level of immunoproteasome in retina was two-fold higher than in brain; CTL-induced retinal injury further up-regulated immunoproteasome expression. Immunoproteasome up-regulation was also observed in injured brain and corresponded with expression in Purkinje cells, microglia, astrocytes, and oligodendrocytes. These results suggest that the normal environment of the retina is sufficiently challenging to require on-going expression of immunoproteasome. Further, immunoproteasome up-regulation with retinal and brain injury implies a role in neuronal protection and/or repair of damage.

MeSH Terms
Animals Brain/immunology,physiopathology Cell Communication/immunology Encephalitis/immunology,physiopathology Mice Mice, Transgenic Nerve Degeneration/immunology,physiopathology Nerve Regeneration/immunology Neuroglia/immunology,pathology Neuronal Plasticity/immunology Neurons/immunology,pathology Pigment Epithelium of Eye/immunology,physiopathology Proteasome Endopeptidase Complex/immunology Protein Subunits/immunology Recovery of Function/immunology Retina/immunology,physiopathology Retinitis/immunology,physiopathology T-Lymphocytes, Cytotoxic/immunology Up-Regulation/immunology
Chemicals
Protein Subunits Proteasome Endopeptidase Complex
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ferrington Deborah A
Department of Ophthalmology, University of Minnesota, Minneapolis, Minnesota, USA. [email protected]
Hussong Stacy A
Roehrich Heidi
Kapphahn Rebecca J
Kavanaugh Shannon M
Heuss Neal D
Gregerson Dale S
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Article Info
Journal
Journal of neurochemistry
Abbr.
J Neurochem
ISSN
1471-4159
DOI
10.1111/j.1471-4159.2008.05345.x
Email
[email protected]
Published
2008-07-00
Epub
2008-00-01
Pages
158-69
Language
English
Region
England
NLM ID
2985190R
PMCID
PMC4401486
Subset
IM
Grants
NIA NIH HHS · R21 AG032391 · United States
NEI NIH HHS · EY011542 · United States
NEI NIH HHS · P30-EY11374 · United States
NEI NIH HHS · P30 EY011374 · United States
NEI NIH HHS · R01 EY013623 · United States
NEI NIH HHS · R01 EY011542 · United States
NEI NIH HHS · EY013623 · United States
External Links
PubMed source DOI: 10.1111/j.1471-4159.2008.05345.x PMC Full Text View journal details
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