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PMID: 1835791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Surface expression of alternative forms of the TCR/CD3 complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10619-23

Kappes DJ, Tonegawa S

Abstract

T-cell antigen receptor (TCR) heterodimers of both the alpha beta and gamma delta types are expressed at the surface of T cells only in association with a complex of invariant chains called CD3. The requirement for individual CD3 components to achieve TCR surface expression was examined by cotransfection of a non-T-cell line with TCR alpha and beta, as well as CD3 delta, epsilon, gamma, and zeta, cDNAs. Both transient and stable transfectants expressing TCR and CD3 epitopes at the cell surface were generated. By transfection of TCR and CD3 components in different combinations, the TCR chains, as well as the CD3 epsilon and zeta chains, were each shown to be essential for reconstituting surface expression. On the other hand, CD3 delta and gamma chains could be used alternatively, providing evidence for two different types of TCR/CD3 complexes.

MeSH Terms
Antibodies, Monoclonal Antigens, CD/genetics Antigens, Differentiation, T-Lymphocyte/analysis,biosynthesis,genetics CD3 Complex Cell Membrane/immunology Electrophoresis, Polyacrylamide Gel Flow Cytometry HeLa Cells Humans Macromolecular Substances Models, Structural Receptors, Antigen, T-Cell/analysis,biosynthesis,genetics Transfection
Chemicals
Antibodies, Monoclonal Antigens, CD Antigens, Differentiation, T-Lymphocyte CD3 Complex Macromolecular Substances Receptors, Antigen, T-Cell
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kappes D J
Howard Hughes Medical Institute, Center for Cancer Research, Cambridge, MA.
Tonegawa S
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10619
Published
1991-12-01
Pages
10619-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52981
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.23.10619 PMC Full Text View journal details
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