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PMID: 18362182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella.

The Journal of cell biology ·Vol. 180 ·No. 6 ·2008-03-24 ·Pages 1233-44

Nemethova M, Auinger S, Small JV

Abstract

Filopodia are rodlike extensions generally attributed with a guidance role in cell migration. We now show in fish fibroblasts that filopodia play a major role in generating contractile bundles in the lamella region behind the migrating front. Filopodia that developed adhesion to the substrate via paxillin containing focal complexes contributed their proximal part to stress fiber assembly, and filopodia that folded laterally contributed to the construction of contractile bundles parallel to the cell edge. Correlated light and electron microscopy of cells labeled for actin and fascin confirmed integration of filopodia bundles into the lamella network. Inhibition of myosin II did not subdue the waving and folding motions of filopodia or their entry into the lamella, but filopodia were not then integrated into contractile arrays. Comparable results were obtained with B16 melanoma cells. These and other findings support the idea that filaments generated in filopodia and lamellipodia for protrusion are recycled for seeding actomyosin arrays for use in retraction.

MeSH Terms
Actins/metabolism Animals Carrier Proteins/metabolism Cell Adhesion/physiology Cell Line Cell Line, Tumor Cell Movement/physiology Cell Shape/physiology Cytoplasmic Streaming/physiology Cytoskeleton/metabolism,ultrastructure Focal Adhesions/metabolism,ultrastructure Goldfish Mice Microfilament Proteins/metabolism Microscopy, Electron Myosin Type II/metabolism Paxillin/metabolism Protein Transport/physiology Pseudopodia/metabolism,ultrastructure Stress, Mechanical
Chemicals
Actins Carrier Proteins Microfilament Proteins Paxillin fascin Myosin Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nemethova Maria
Institute of Molecular Biotechnology, Austrian Academy of Sciences, Vienna 1030, Austria.
Auinger Sonja
Small J Victor
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200709134
Published
2008-03-24
Pages
1233-44
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2290848
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200709134 PMC Full Text View journal details
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