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PMID: 18390669 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

MicroRNA-repressed mRNAs contain 40S but not 60S components.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 105 ·No. 14 ·2008-04-08 ·Pages 5343-8

Wang B, Yanez A, Novina CD

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that may target more than one-third of human genes, yet the mechanisms used by miRNAs to repress translation of target mRNAs are obscure. Using a recently described cell-free assay of miRNA function, we observe that miRNA-targeted mRNAs are enriched for 40S but not 60S ribosome components. Additionally, toeprinting analysis of miRNA-targeted mRNAs demonstrates that approximately 18 nt 3' relative to the initiating AUG are protected, consistent with 40S ribosome subunits positioned at the AUG codon. Our results suggest that miRNAs repress translation initiation by preventing 60S subunit joining to miRNA-targeted mRNAs.

MeSH Terms
Codon, Initiator/antagonists & inhibitors Humans MicroRNAs/genetics Peptide Chain Initiation, Translational RNA, Messenger/antagonists & inhibitors,genetics Ribosomes/genetics
Chemicals
Codon, Initiator MicroRNAs RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang Bingbing
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
Yanez Adrienne
Novina Carl D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0801102105
Published
2008-04-08
Epub
2008-00-04
Pages
5343-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2291078
Subset
IM
Grants
NIGMS NIH HHS · F31 GM087947 · United States
NIGMS NIH HHS · T32 GM007266 · United States
NIGMS NIH HHS · GM07266 · United States
External Links
PubMed source DOI: 10.1073/pnas.0801102105 PMC Full Text View journal details
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