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PMID: 18401012 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Extracellular adenosine 5'-triphosphate alters motility and improves the fertilizing capability of mouse sperm.

Biology of reproduction ·Vol. 79 ·No. 1 ·2008-00-00 ·Pages 164-71

Rodríguez-Miranda E, Buffone MG, Edwards SE, Ord TS, Lin K, Sammel MD, Gerton GL, Moss SB, Williams CJ

Abstract

Extracellular adenosine 5'-triphosphate (ATPe) treatment of human sperm has been implicated in improving in vitro fertilization (IVF) results. We used the mouse model to investigate mechanisms of action of ATPe on sperm. ATPe treatment significantly enhanced IVF success as indicated by both rate of pronuclear formation and percentage cleavage to the 2-cell stage. However, ATPe did not increase the percentage of sperm undergoing spontaneous acrosomal exocytosis nor change the pattern of protein tyrosine phosphorylation normally observed in capacitated sperm. ATPe altered sperm motility parameters; in particular, both noncapacitated and capacitated sperm swam faster and straighter. The percentage of hyperactivated sperm did not increase in capacitated ATPe-treated sperm compared to control sperm. ATPe induced a rapid increase in the level of intracellular calcium that was inhibited by two distinct P2 purinergic receptor inhibitors, confirming that these receptors have an ionotropic role in sperm function. The observed motility changes likely explain, in part, the improved fertilizing capability when ATPe-treated sperm were used in IVF procedures and suggest a mechanism by which ATPe treatment may be beneficial for artificial reproductive techniques.

MeSH Terms
Acrosome/drug effects,metabolism Adenosine Triphosphate/metabolism,pharmacology Animals Calcium Signaling/drug effects Drug Evaluation, Preclinical Extracellular Space/metabolism Female Fertilization/drug effects Fertilization in Vitro/drug effects Male Mice Mice, Inbred C57BL Mice, Inbred Strains Phosphorylation/drug effects Protein-Tyrosine Kinases/metabolism Sperm Capacitation/drug effects Sperm Motility/drug effects Tyrosine/metabolism
Chemicals
Tyrosine Adenosine Triphosphate Protein-Tyrosine Kinases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Rodríguez-Miranda Esmeralda
Department of Obstetrics & Gynecology, Center for Research on Reproduction & Women's Health, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Buffone Mariano G
Edwards Scott E
Ord Teri S
Lin Kathleen
Sammel Mary D
Gerton George L
Moss Stuart B
Williams Carmen J
References (33)
33 references, click to expand
  1. Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation.
    Development. 1995 Apr;121(4):1129-37 PMID: 7743926
  2. A new sperm-specific Na+/H+ exchanger required for sperm motility and fertility.
    Nat Cell Biol. 2003 Dec;5(12):1117-22 PMID: 14634667
  3. Design and pharmacology of selective P2-purinoceptor antagonists.
    J Auton Pharmacol. 1996 Dec;16(6):341-4 PMID: 9131412
  4. Asthenozoospermia: analysis of a large population.
    Arch Androl. 2003 Sep-Oct;49(5):343-9 PMID: 12893510
  5. Roles of cAMP in regulating microtubule sliding and flagellar bending in demembranated hamster spermatozoa.
    FEBS Lett. 2006 Feb 20;580(5):1515-20 PMID: 16466717
  6. Kinetic properties of "soluble" adenylyl cyclase. Synergism between calcium and bicarbonate.
    J Biol Chem. 2003 May 2;278(18):15922-6 PMID: 12609998
  7. Hyperactivated motility of bull sperm is triggered at the axoneme by Ca2+ and not cAMP.
    Dev Biol. 2002 Oct 1;250(1):208-17 PMID: 12297107
  8. CATSPER channel-mediated Ca2+ entry into mouse sperm triggers a tail-to-head propagation.
    Biol Reprod. 2007 Sep;77(3):551-9 PMID: 17554080
  9. Adenosine triphosphate induces Ca2+ signal in epithelial cells of the mouse caput epididymis through activation of P2X and P2Y purinergic receptors.
    Biol Reprod. 2003 Apr;68(4):1185-92 PMID: 12606441
  10. Adenylate kinases 1 and 2 are part of the accessory structures in the mouse sperm flagellum.
    Biol Reprod. 2006 Oct;75(4):492-500 PMID: 16790685
  11. Sperm-specific protein kinase A catalytic subunit Calpha2 orchestrates cAMP signaling for male fertility.
    Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13483-8 PMID: 15340140
  12. Regulation of protein tyrosine phosphorylation in human sperm by a calcium/calmodulin-dependent mechanism: identification of A kinase anchor proteins as major substrates for tyrosine phosphorylation.
    Dev Biol. 1996 Nov 25;180(1):284-96 PMID: 8948591
  13. Effects of extracellular adenosine 5'-triphosphate on human sperm motility.
    Reprod Sci. 2007 Oct;14(7):655-66 PMID: 18000227
  14. Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein.
    FEBS Lett. 1999 Apr 23;449(2-3):277-83 PMID: 10338148
  15. Purinergic signalling.
    Br J Pharmacol. 2006 Jan;147 Suppl 1:S172-81 PMID: 16402102
  16. Protein phosphorylation in mammalian spermatozoa.
    Reproduction. 2003 Jan;125(1):17-26 PMID: 12622692
  17. Maturation-associated increase in IP3 receptor type 1: role in conferring increased IP3 sensitivity and Ca2+ oscillatory behavior in mouse eggs.
    Dev Biol. 2003 Feb 15;254(2):163-71 PMID: 12591238
  18. Functional cross talk after activation of P2 and P1 receptors in oviductal ciliated cells.
    Am J Physiol Cell Physiol. 2000 Sep;279(3):C658-69 PMID: 10942716
  19. Mice deficient in CHRNA7, a subunit of the nicotinic acetylcholine receptor, produce sperm with impaired motility.
    Biol Reprod. 2005 Oct;73(4):807-14 PMID: 15944242
  20. Mechanism of human sperm activation by extracellular ATP.
    Am J Physiol. 1996 Jun;270(6 Pt 1):C1709-14 PMID: 8764154
  21. PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses.
    Eur J Pharmacol. 1992 Jul 7;217(2-3):217-9 PMID: 1330591
  22. Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility.
    Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16501-6 PMID: 15546993
  23. Insights into sperm cell motility signaling through sNHE and the CatSpers.
    Mol Cell Endocrinol. 2006 May 16;250(1-2):84-92 PMID: 16413670
  24. Receptors for purines and pyrimidines.
    Pharmacol Rev. 1998 Sep;50(3):413-92 PMID: 9755289
  25. Signaling pathways for modulation of mouse sperm motility by adenosine and catecholamine agonists.
    Biol Reprod. 2006 Mar;74(3):492-500 PMID: 16291925
  26. Acrosome-reacted human sperm in insemination medium do not bind to the zona pellucida of human oocytes.
    Int J Androl. 2006 Aug;29(4):475-81 PMID: 16480409
  27. Extracellular adenosine triphosphate stimulates acrosomal exocytosis in bovine spermatozoa via P2 purinoceptor.
    Biol Reprod. 2002 Feb;66(2):429-37 PMID: 11804959
  28. Pharmacological profiles of cloned mammalian P2Y-receptor subtypes.
    Pharmacol Ther. 2006 Jun;110(3):415-32 PMID: 16257449
  29. Contributions of extracellular and intracellular Ca2+ to regulation of sperm motility: Release of intracellular stores can hyperactivate CatSper1 and CatSper2 null sperm.
    Dev Biol. 2007 Mar 1;303(1):214-21 PMID: 17174296
  30. Extracellular ATP is a trigger for the acrosome reaction in human spermatozoa.
    J Biol Chem. 1992 Sep 25;267(27):19443-7 PMID: 1527064
  31. Sperm treatment with extracellular ATP increases fertilization rates in in-vitro fertilization for male factor infertility.
    Hum Reprod. 1999 Mar;14(3):694-7 PMID: 10221697
  32. Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+].
    Biophys J. 1989 Dec;56(6):1255-8 PMID: 2611335
  33. Reduced vas deferens contraction and male infertility in mice lacking P2X1 receptors.
    Nature. 2000 Jan 6;403(6765):86-9 PMID: 10638758
Article Info
Journal
Biology of reproduction
Abbr.
Biol Reprod
ISSN
0006-3363
DOI
10.1095/biolreprod.107.065565
Published
2008-00-00
Epub
2008-00-09
Pages
164-71
Language
English
Region
United States
NLM ID
0207224
PMCID
PMC6280785
Subset
IM
Grants
FIC NIH HHS · D43-TW00671 · United States
NICHD NIH HHS · HD41552 · United States
External Links
PubMed source DOI: 10.1095/biolreprod.107.065565 PMC Full Text View journal details
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