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PMID: 18402783 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome.

Biochimica et biophysica acta ·Vol. 1784 ·No. 7-8 ·2008-00-00 ·Pages 983-5

Gilany K, Van Elzen R, Mous K, Coen E, Van Dongen W, Vandamme S, Gevaert K, Timmerman E, Vandekerckhove J, Dewilde S, Van Ostade X, Moens L

Abstract

The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, N-terminal COFRADIC analysis with nano-LC-MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.

MeSH Terms

Cell Line, Tumor Electrophoresis, Polyacrylamide Gel Humans Neoplasm Proteins/metabolism Neuroblastoma/metabolism,pathology Proteome Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Chemicals

Neoplasm Proteins Proteome

Authors & Affiliations

12 authors, click to expand affiliations / ORCID

Gilany Kambiz

Department of Biomedical Sciences, Protein Chemistery, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.

Van Elzen Roos

Mous Kim

Coen Edmond

Van Dongen Walter

Vandamme Stefaan

Gevaert Kris

Timmerman Evy

Vandekerckhove Joël

Dewilde Sylvia

Van Ostade Xaveer

Moens Luc

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/j.bbapap.2008.03.003

Published

2008-00-00

Epub

2008-00-19

Pages

983-5

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/j.bbapap.2008.03.003

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The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome.

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