Home LiteratureArticle Details
PMID: 18407065 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome.

Cell host & microbe ·Vol. 3 ·No. 4 ·2008-04-17 ·Pages 213-23

Turnbaugh PJ, Bäckhed F, Fulton L, Gordon JI

Abstract

We have investigated the interrelationship between diet, gut microbial ecology, and energy balance using a mouse model of obesity produced by consumption of a prototypic Western diet. Diet-induced obesity (DIO) produced a bloom in a single uncultured clade within the Mollicutes class of the Firmicutes, which was diminished by subsequent dietary manipulations that limit weight gain. Microbiota transplantation from mice with DIO to lean germ-free recipients promoted greater fat deposition than transplants from lean donors. Metagenomic and biochemical analysis of the gut microbiome together with sequencing and metabolic reconstructions of a related human gut-associated Mollicute (Eubacterium dolichum) revealed features that may provide a competitive advantage to members of the bloom in the Western diet nutrient milieu, including import and processing of simple sugars. Our study illustrates how combining comparative metagenomics with gnotobiotic mouse models and specific dietary manipulations can disclose the niches of previously uncharacterized members of the gut microbiota.

MeSH Terms
Animals Bacteria/classification,genetics,isolation & purification Carbohydrate Metabolism Carbohydrates/administration & dosage,adverse effects Cecum/microbiology Diet/adverse effects Disease Models, Animal Energy Metabolism Eubacterium/genetics,isolation & purification Fats/administration & dosage,adverse effects Male Mice Mice, Inbred C57BL Mice, Obese Molecular Sequence Data Obesity/chemically induced,metabolism,microbiology RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Specific Pathogen-Free Organisms Tenericutes/classification,genetics,isolation & purification
Chemicals
Carbohydrates Fats RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Turnbaugh Peter J
Center for Genome Sciences, Washington University, St. Louis, MO 63108, USA.
Bäckhed Fredrik
Fulton Lucinda
Gordon Jeffrey I
References (33)
33 references, click to expand
  1. Genome streamlining in a cosmopolitan oceanic bacterium.
    Science. 2005 Aug 19;309(5738):1242-5 PMID: 16109880
  2. Mechanisms underlying the resistance to diet-induced obesity in germ-free mice.
    Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):979-84 PMID: 17210919
  3. The gut microbiota as an environmental factor that regulates fat storage.
    Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15718-23 PMID: 15505215
  4. Use of simulated data sets to evaluate the fidelity of metagenomic processing methods.
    Nat Methods. 2007 Jun;4(6):495-500 PMID: 17468765
  5. Host-mediated inflammation disrupts the intestinal microbiota and promotes the overgrowth of Enterobacteriaceae.
    Cell Host Microbe. 2007 Aug 16;2(2):119-29 PMID: 18005726
  6. Aberrant expansion of segmented filamentous bacteria in IgA-deficient gut.
    Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1981-6 PMID: 14766966
  7. Cytoskeletal protein P41 is required to anchor the terminal organelle of the wall-less prokaryote Mycoplasma pneumoniae.
    Mol Microbiol. 2007 Jan;63(1):44-53 PMID: 17163973
  8. MODELTEST: testing the model of DNA substitution.
    Bioinformatics. 1998;14(9):817-8 PMID: 9918953
  9. The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis.
    Nucleic Acids Res. 2005 Jan 1;33(Database issue):D294-6 PMID: 15608200
  10. How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
    Microbiol Mol Biol Rev. 2006 Dec;70(4):939-1031 PMID: 17158705
  11. Diversity of the human intestinal microbial flora.
    Science. 2005 Jun 10;308(5728):1635-8 PMID: 15831718
  12. Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness.
    Appl Environ Microbiol. 2005 Mar;71(3):1501-6 PMID: 15746353
  13. An obesity-associated gut microbiome with increased capacity for energy harvest.
    Nature. 2006 Dec 21;444(7122):1027-31 PMID: 17183312
  14. Microbial ecology: human gut microbes associated with obesity.
    Nature. 2006 Dec 21;444(7122):1022-3 PMID: 17183309
  15. UniFrac--an online tool for comparing microbial community diversity in a phylogenetic context.
    BMC Bioinformatics. 2006 Aug 07;7:371 PMID: 16893466
  16. Respiratory uncoupling lowers blood pressure through a leptin-dependent mechanism in genetically obese mice.
    Arterioscler Thromb Vasc Biol. 2002 Jun 1;22(6):961-8 PMID: 12067905
  17. Metabolic profiling reveals a contribution of gut microbiota to fatty liver phenotype in insulin-resistant mice.
    Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12511-6 PMID: 16895997
  18. Glycan foraging in vivo by an intestine-adapted bacterial symbiont.
    Science. 2005 Mar 25;307(5717):1955-9 PMID: 15790854
  19. Obesity alters gut microbial ecology.
    Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11070-5 PMID: 16033867
  20. ARACHNE: a whole-genome shotgun assembler.
    Genome Res. 2002 Jan;12(1):177-89 PMID: 11779843
  21. Congenital leptin deficiency is associated with severe early-onset obesity in humans.
    Nature. 1997 Jun 26;387(6636):903-8 PMID: 9202122
  22. IgA response to symbiotic bacteria as a mediator of gut homeostasis.
    Cell Host Microbe. 2007 Nov 15;2(5):328-39 PMID: 18005754
  23. MEGAN analysis of metagenomic data.
    Genome Res. 2007 Mar;17(3):377-86 PMID: 17255551
  24. InterPro, progress and status in 2005.
    Nucleic Acids Res. 2005 Jan 1;33(Database issue):D201-5 PMID: 15608177
  25. Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases.
    Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13780-5 PMID: 17699621
  26. MetaGene: prokaryotic gene finding from environmental genome shotgun sequences.
    Nucleic Acids Res. 2006;34(19):5623-30 PMID: 17028096
  27. STRING 7--recent developments in the integration and prediction of protein interactions.
    Nucleic Acids Res. 2007 Jan;35(Database issue):D358-62 PMID: 17098935
  28. Ecological and evolutionary forces shaping microbial diversity in the human intestine.
    Cell. 2006 Feb 24;124(4):837-48 PMID: 16497592
  29. The complete genome and proteome of Mycoplasma mobile.
    Genome Res. 2004 Aug;14(8):1447-61 PMID: 15289470
  30. Reduced dietary intake of carbohydrates by obese subjects results in decreased concentrations of butyrate and butyrate-producing bacteria in feces.
    Appl Environ Microbiol. 2007 Feb;73(4):1073-8 PMID: 17189447
  31. The KEGG resource for deciphering the genome.
    Nucleic Acids Res. 2004 Jan 1;32(Database issue):D277-80 PMID: 14681412
  32. A top-down systems biology view of microbiome-mammalian metabolic interactions in a mouse model.
    Mol Syst Biol. 2007;3:112 PMID: 17515922
  33. An application of statistics to comparative metagenomics.
    BMC Bioinformatics. 2006 Mar 20;7:162 PMID: 16549025
Article Info
Journal
Cell host & microbe
Abbr.
Cell Host Microbe
ISSN
1934-6069
DOI
10.1016/j.chom.2008.02.015
Published
2008-04-17
Pages
213-23
Language
English
Region
United States
NLM ID
101302316
PMCID
PMC3687783
Subset
IM
Grants
NIDDK NIH HHS · P01 DK078669 · United States
NIDDK NIH HHS · R01 DK030292 · United States
NIDDK NIH HHS · DK30292 · United States
NIDDK NIH HHS · DK70977 · United States
NIDDK NIH HHS · R37 DK030292 · United States
NIDDK NIH HHS · R01 DK070977 · United States
NIDDK NIH HHS · P30 DK056341-08 · United States
NIDDK NIH HHS · P30 DK056341 · United States
NIDDK NIH HHS · P30 DK056341-07 · United States
Databases
GENBANK
EU503541, EU503542, EU503543, EU503544, EU503545, EU503546, EU503547, EU503548, EU503549, EU503550, EU503551, EU503552, EU503553, EU503554, EU503555, EU503556, EU503557, EU503558, EU503559, EU503560, EU503561, EU503562, EU503563, EU503564, EU503565, EU503566, EU503567, EU503568, EU503569, EU503570, EU503571, EU503572, EU503573, EU503574, EU503575, EU503576, EU503577, EU503578, EU503579, EU503580, EU503581, EU503582, EU503583, EU503584, EU503585, EU503586, EU503587, EU503588, EU503589, EU503590, EU503591, EU503592, EU503593, EU503594, EU503595, EU503596, EU503597, EU503598, EU503599, EU503600, EU503601, EU503602, EU503603, EU503604, EU503605, EU503606, EU503607, EU503608, EU503609, EU503610, EU503611, EU503612, EU503613, EU503614, EU503615, EU503616, EU503617, EU503618, EU503619, EU503620, EU503621, EU503622, EU503623, EU503624, EU503625, EU503626, EU503627, EU503628, EU503629, EU503630, EU503631, EU503632, EU503633, EU503634, EU503635, EU503636, EU503637, EU503638, EU503639, EU503640, EU503641, EU503642, EU503643, EU503644, EU503645, EU503646, EU503647, EU503648, EU503649, EU503650, EU503651, EU503652, EU503653, EU503654, EU503655, EU503656, EU503657, EU503658, EU503659, EU503660, EU503661, EU503662, EU503663, EU503664, EU503665, EU503666, EU503667, EU503668, EU503669, EU503670, EU503671, EU503672, EU503673, EU503674, EU503675, EU503676, EU503677, EU503678, EU503679, EU503680, EU503681, EU503682, EU503683, EU503684, EU503685, EU503686, EU503687, EU503688, EU503689, EU503690, EU503691, EU503692, EU503693, EU503694, EU503695, EU503696, EU503697, EU503698, EU503699, EU503700, EU503701, EU503702, EU503703, EU503704, EU503705, EU503706, EU503707, EU503708, EU503709, EU503710, EU503711, EU503712, EU503713, EU503714, EU503715, EU503716, EU503717, EU503718, EU503719, EU503720, EU503721, EU503722, EU503723, EU503724, EU503725, EU503726, EU503727, EU503728, EU503729, EU503730, EU503731, EU503732, EU503733, EU503734, EU503735, EU503736, EU503737, EU503738, EU503739, EU503740, EU503741, EU503742, EU503743, EU503744, EU503745, EU503746, EU503747, EU503748, EU503749, EU503750, EU503751, EU503752, EU503753, EU503754, EU503755, EU503756, EU503757, EU503758, EU503759, EU503760, EU503761, EU503762, EU503763, EU503764, EU503765, EU503766, EU503767, EU503768, EU503769, EU503770, EU503771, EU503772, EU503773, EU503774, EU503775, EU503776, EU503777, EU503778, EU503779, EU503780, EU503781, EU503782, EU503783, EU503784, EU503785, EU503786, EU503787, EU503788, EU503789, EU503790, EU503791, EU503792, EU503793, EU503794, EU503795, EU503796, EU503797, EU503798, EU503799, EU503800, EU503801, EU503802, EU503803, EU503804, EU503805, EU503806, EU503807, EU503808, EU503809, EU503810, EU503811, EU503812, EU503813, EU503814, EU503815, EU503816, EU503817, EU503818, EU503819, EU503820, EU503821, EU503822, EU503823, EU503824, EU503825, EU503826, EU503827, EU503828, EU503829, EU503830, EU503831, EU503832, EU503833, EU503834, EU503835, EU503836, EU503837, EU503838, EU503839, EU503840, EU503841, EU503842, EU503843, EU503844, EU503845, EU503846, EU503847, EU503848, EU503849, EU503850, EU503851, EU503852, EU503853, EU503854, EU503855, EU503856, EU503857, EU503858, EU503859, EU503860, EU503861, EU503862, EU503863, EU503864, EU503865, EU503866, EU503867, EU503868, EU503869, EU503870, EU503871, EU503872, EU503873, EU503874, EU503875, EU503876, EU503877, EU503878, EU503879, EU503880, EU503881, EU503882, EU503883, EU503884, EU503885, EU503886, EU503887, EU503888, EU503889, EU503890, EU503891, EU503892, EU503893, EU503894, EU503895, EU503896, EU503897, EU503898, EU503899, EU503900, EU503901, EU503902, EU503903, EU503904, EU503905, EU503906, EU503907, EU503908, EU503909, EU503910, EU503911, EU503912, EU503913, EU503914, EU503915, EU503916, EU503917, EU503918, EU503919, EU503920, EU503921, EU503922, EU503923, EU503924, EU503925, EU503926, EU503927, EU503928, EU503929, EU503930, EU503931, EU503932, EU503933, EU503934, EU503935, EU503936, EU503937, EU503938, EU503939, EU503940, EU503941, EU503942, EU503943, EU503944, EU503945, EU503946, EU503947, EU503948, EU503949, EU503950, EU503951, EU503952, EU503953, EU503954, EU503955, EU503956, EU503957, EU503958, EU503959, EU503960, EU503961, EU503962, EU503963, EU503964, EU503965, EU503966, EU503967, EU503968, EU503969, EU503970, EU503971, EU503972, EU503973, EU503974, EU503975, EU503976, EU503977, EU503978, EU503979, EU503980, EU503981, EU503982, EU503983, EU503984, EU503985, EU503986, EU503987, EU503988, EU503989, EU503990, EU503991, EU503992, EU503993, EU503994, EU503995, EU503996, EU503997, EU503998, EU503999, EU504000, EU504001, EU504002, EU504003, EU504004, EU504005, EU504006, EU504007, EU504008, EU504009, EU504010, EU504011, EU504012, EU504013, EU504014, EU504015, EU504016, EU504017, EU504018, EU504019, EU504020, EU504021, EU504022, EU504023, EU504024, EU504025, EU504026, EU504027, EU504028, EU504029, EU504030, EU504031, EU504032, EU504033, EU504034, EU504035, EU504036, EU504037, EU504038, EU504039, EU504040, EU504041, EU504042, EU504043, EU504044, EU504045, EU504046, EU504047, EU504048, EU504049, EU504050, EU504051, EU504052, EU504053, EU504054, EU504055, EU504056, EU504057, EU504058, EU504059, EU504060, EU504061, EU504062, EU504063, EU504064, EU504065, EU504066, EU504067, EU504068, EU504069, EU504070, EU504071, EU504072, EU504073, EU504074, EU504075, EU504076, EU504077, EU504078, EU504079, EU504080, EU504081, EU504082, EU504083, EU504084, EU504085, EU504086, EU504087, EU504088, EU504089, EU504090, EU504091, EU504092, EU504093, EU504094, EU504095, EU504096, EU504097, EU504098, EU504099, EU504100, EU504101, EU504102, EU504103, EU504104, EU504105, EU504106, EU504107, EU504108, EU504109, EU504110, EU504111, EU504112, EU504113, EU504114, EU504115, EU504116, EU504117, EU504118, EU504119, EU504120, EU504121, EU504122, EU504123, EU504124, EU504125, EU504126, EU504127, EU504128, EU504129, EU504130, EU504131, EU504132, EU504133, EU504134, EU504135, EU504136, EU504137, EU504138, EU504139, EU504140, EU504141, EU504142, EU504143, EU504144, EU504145, EU504146, EU504147, EU504148, EU504149, EU504150, EU504151, EU504152, EU504153, EU504154, EU504155, EU504156, EU504157, EU504158, EU504159, EU504160, EU504161, EU504162, EU504163, EU504164, EU504165, EU504166, EU504167, EU504168, EU504169, EU504170, EU504171, EU504172, EU504173, EU504174, EU504175, EU504176, EU504177, EU504178, EU504179, EU504180, EU504181, EU504182, EU504183, EU504184, EU504185, EU504186, EU504187, EU504188, EU504189, EU504190, EU504191, EU504192, EU504193, EU504194, EU504195, EU504196, EU504197, EU504198, EU504199, EU504200, EU504201, EU504202, EU504203, EU504204, EU504205, EU504206, EU504207, EU504208, EU504209, EU504210, EU504211, EU504212, EU504213, EU504214, EU504215, EU504216, EU504217, EU504218, EU504219, EU504220, EU504221, EU504222, EU504223, EU504224, EU504225, EU504226, EU504227, EU504228, EU504229, EU504230, EU504231, EU504232, EU504233, EU504234, EU504235, EU504236, EU504237, EU504238, EU504239, EU504240, EU504241, EU504242, EU504243, EU504244, EU504245, EU504246, EU504247, EU504248, EU504249, EU504250, EU504251, EU504252, EU504253, EU504254, EU504255, EU504256, EU504257, EU504258, EU504259, EU504260, EU504261, EU504262, EU504263, EU504264, EU504265, EU504266, EU504267, EU504268, EU504269, EU504270, EU504271, EU504272, EU504273, EU504274, EU504275, EU504276, EU504277, EU504278, EU504279, EU504280, EU504281, EU504282, EU504283, EU504284, EU504285, EU504286, EU504287, EU504288, EU504289, EU504290, EU504291, EU504292, EU504293, EU504294, EU504295, EU504296, EU504297, EU504298, EU504299, EU504300, EU504301, EU504302, EU504303, EU504304, EU504305, EU504306, EU504307, EU504308, EU504309, EU504310, EU504311, EU504312, EU504313, EU504314, EU504315, EU504316, EU504317, EU504318, EU504319, EU504320, EU504321, EU504322, EU504323, EU504324, EU504325, EU504326, EU504327, EU504328, EU504329, EU504330, EU504331, EU504332, EU504333, EU504334, EU504335, EU504336, EU504337, EU504338, EU504339, EU504340, EU504341, EU504342, EU504343, EU504344, EU504345, EU504346, EU504347, EU504348, EU504349, EU504350, EU504351, EU504352, EU504353, EU504354, EU504355, EU504356, EU504357, EU504358, EU504359, EU504360, EU504361, EU504362, EU504363, EU504364, EU504365, EU504366, EU504367, EU504368, EU504369, EU504370, EU504371, EU504372, EU504373, EU504374, EU504375, EU504376, EU504377, EU504378, EU504379, EU504380, EU504381, EU504382, EU504383, EU504384, EU504385, EU504386, EU504387, EU504388, EU504389, EU504390, EU504391, EU504392, EU504393, EU504394, EU504395, EU504396, EU504397, EU504398, EU504399, EU504400, EU504401, EU504402, EU504403, EU504404, EU504405, EU504406, EU504407, EU504408, EU504409, EU504410, EU504411, EU504412, EU504413, EU504414, EU504415, EU504416, EU504417, EU504418, EU504419, EU504420, EU504421, EU504422, EU504423, EU504424, EU504425, EU504426, EU504427, EU504428, EU504429, EU504430, EU504431, EU504432, EU504433, EU504434, EU504435, EU504436, EU504437, EU504438, EU504439, EU504440, EU504441, EU504442, EU504443, EU504444, EU504445, EU504446, EU504447, EU504448, EU504449, EU504450, EU504451, EU504452, EU504453, EU504454, EU504455, EU504456, EU504457, EU504458, EU504459, EU504460, EU504461, EU504462, EU504463, EU504464, EU504465, EU504466, EU504467, EU504468, EU504469, EU504470, EU504471, EU504472, EU504473, EU504474, EU504475, EU504476, EU504477, EU504478, EU504479, EU504480, EU504481, EU504482, EU504483, EU504484, EU504485, EU504486, EU504487, EU504488, EU504489, EU504490, EU504491, EU504492, EU504493, EU504494, EU504495, EU504496, EU504497, EU504498, EU504499, EU504500, EU504501, EU504502, EU504503, EU504504, EU504505, EU504506, EU504507, EU504508, EU504509, EU504510, EU504511, EU504512, EU504513, EU504514, EU504515, EU504516, EU504517, EU504518, EU504519, EU504520, EU504521, EU504522, EU504523, EU504524, EU504525, EU504526, EU504527, EU504528, EU504529, EU504530, EU504531, EU504532, EU504533, EU504534, EU504535, EU504536, EU504537, EU504538, EU504539, EU504540
External Links
PubMed source DOI: 10.1016/j.chom.2008.02.015 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2