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PMID: 1840919 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Differential activation of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes by wounding and pathogen challenge.

The Plant cell ·Vol. 3 ·No. 4 ·1991-04-00 ·Pages 397-405

Yang Z, Park H, Lacy GH, Cramer CL

Abstract

Potato genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) were expressed in response to pathogen, elicitor, and wounding. HMGR catalyzes the rate-limiting step in isoprenoid biosynthesis leading to accumulation of phytoalexins and steroid glycoalkaloids. Wounding caused increases in HMGR mRNA levels. A rapid and transient peak occurred 30 minutes after wounding, followed by a slower peak at 14 hours; both were correlated with increased enzyme activity. Induction of HMGR mRNA by the soft rot pathogen Erwinia carotovora subsp carotovora or arachidonic acid began 8 hours after challenge and continued through 22 hours. Potato HMGR is encoded by a gene family. An HMGR gene-specific probe was used to demonstrate that one isogene of the HMGR family is pathogen activated and is distinct from isogene(s) that are wound activated. This provides evidence that defense-related increases in HMGR activity are due to mRNA level increases and that HMGR isogenes are activated differentially by wounding or pathogen challenge.

MeSH Terms
Arachidonic Acids/pharmacology Gene Expression Regulation Hydroxymethylglutaryl CoA Reductases/genetics,metabolism Isoproterenol/metabolism Kinetics Multigene Family Pectobacterium carotovorum/physiology Solanum tuberosum/enzymology,genetics,microbiology
Chemicals
Arachidonic Acids Hydroxymethylglutaryl CoA Reductases Isoproterenol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang Z
Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg 24061-0330.
Park H
Lacy G H
Cramer C L
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.3.4.397
Published
1991-04-00
Pages
397-405
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160009
Subset
IM
Grants
NIGMS NIH HHS · R29GM39549 · United States
External Links
PubMed source DOI: 10.1105/tpc.3.4.397 PMC Full Text View journal details
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