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PMID: 1841710 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Measurement of 15N-13C J couplings in staphylococcal nuclease.

Journal of biomolecular NMR ·Vol. 1 ·No. 4 ·1991-11-00 ·Pages 439-46

Delaglio F, Torchia DA, Bax A

Abstract

15N-C alpha and 15N-C' J couplings were measured for the backbone of staphylococcal nuclease, uniformly enriched with 15N and 13C. It is found that the 1JC'N coupling is similar for beta-sheet, J = 14.8 +/- 0.5 and for alpha-helix, J = 14.8 +/- 0.4 but tends to be larger for the unstructured N- and C-terminal ends of the protein (J = 15.6 +/- 0.5). On average, 1JNC alpha are smaller for alpha-helical residues (J = 9.6 +/- 0.3 Hz) compared to beta-sheet (J = 10.9 +/- 0.8 Hz) and a substantial difference is observed for 2JNC alpha in alpha-helices (J = 6.4 +/- 0.4 Hz) and beta-sheets (J = 8.3 +/- 0.8 Hz).

MeSH Terms
Amino Acid Sequence Carbon Isotopes Magnetic Resonance Spectroscopy/methods Mathematics Micrococcal Nuclease/chemistry Molecular Sequence Data Nitrogen Isotopes Protein Conformation
Chemicals
Carbon Isotopes Nitrogen Isotopes Micrococcal Nuclease
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Delaglio F
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Torchia D A
Bax A
References (8)
8 references, click to expand
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Article Info
Journal
Journal of biomolecular NMR
Abbr.
J Biomol NMR
ISSN
0925-2738
DOI
10.1007/BF02192865
Published
1991-11-00
Pages
439-46
Language
English
Region
Netherlands
NLM ID
9110829
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02192865 View journal details
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