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PMID: 184457 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fate of mismatched base-pair regions in polyoma heteroduplex DNA during infection of mouse cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3073-7

Miller LK, Cooke BE, Fried M

Abstract

Heteroduplex DNA has been constructed from two variants of polyoma virus that differ genotypically at four distinct sites. The genotypes of the progeny virus derived from infections of mouse cells with single heteroduplexes have been analyzed to determine how the genotypic markers of the parental heteroduplex segregate. Markers that are separated by a length of DNA greater than 600 nucleotides segregate independently. Segregation was not detected between two markers separated by only approximately 90 nucleotides. We interpret these results on the basis of the correction of mismatched base-pair regions in the heteroduplex before the completion of DNA replication. We suggest that this technique provides valuable information concerning gene conversion in mamalian cells and permits the transfer of genotypic markers from one virus strain to another.

MeSH Terms
Base Sequence Chromosome Mapping DNA Repair DNA Replication DNA Restriction Enzymes DNA, Viral/analysis,metabolism Genotype Nucleic Acid Renaturation Polyomavirus/metabolism
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller L K
Cooke B E
Fried M
References (22)
22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.9.3073
Published
1976-09-00
Pages
3073-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430933
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.9.3073 PMC Full Text View journal details
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