Home LiteratureArticle Details
PMID: 18446154 Published · ppublish English Letter Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Unexpected failure rates for modular assembly of engineered zinc fingers.

Nature methods ·Vol. 5 ·No. 5 ·2008-05-00 ·Pages 374-5

Ramirez CL, Foley JE, Wright DA, Müller-Lerch F, Rahman SH, Cornu TI, Winfrey RJ, Sander JD, Fu F, Townsend JA, Cathomen T, Voytas DF, Joung JK

Abstract

暂无摘要

MeSH Terms
Cell Line DNA/metabolism Deoxyribonucleases/chemistry,metabolism Genetic Engineering/methods Humans Two-Hybrid System Techniques Zinc Fingers
Chemicals
DNA Deoxyribonucleases
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Ramirez Cherie L
Foley Jonathan E
Wright David A
Müller-Lerch Felix
Rahman Shamim H
Cornu Tatjana I
Winfrey Ronnie J
Sander Jeffry D
Fu Fengli
Townsend Jeffrey A
Cathomen Toni
Voytas Daniel F
Joung J Keith
References (6)
6 references, click to expand
  1. Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly.
    Nat Protoc. 2006;1(3):1637-52 PMID: 17406455
  2. Gene targeting using zinc finger nucleases.
    Nat Biotechnol. 2005 Aug;23(8):967-73 PMID: 16082368
  3. Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins.
    Biochemistry. 2003 Feb 25;42(7):2137-48 PMID: 12590603
  4. Zinc Finger Tools: custom DNA-binding domains for transcription factors and nucleases.
    Nucleic Acids Res. 2006 Jul 1;34(Web Server issue):W516-23 PMID: 16845061
  5. Validated zinc finger protein designs for all 16 GNN DNA triplet targets.
    J Biol Chem. 2002 Feb 8;277(6):3850-6 PMID: 11726671
  6. Human zinc fingers as building blocks in the construction of artificial transcription factors.
    Nat Biotechnol. 2003 Mar;21(3):275-80 PMID: 12592413
Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth0508-374
Published
2008-05-00
Pages
374-5
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC7880305
Subset
IM
Grants
NIH HHS · DP1 OD006862 · United States
NCRR NIH HHS · R21 RR024189 · United States
Corrections
ErratumIn
-
CommentIn
PMID: 20111032
External Links
PubMed source DOI: 10.1038/nmeth0508-374 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2