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PMID: 18458058 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Nuclear factor 45 (NF45) is a regulatory subunit of complexes with NF90/110 involved in mitotic control.

Molecular and cellular biology ·Vol. 28 ·No. 14 ·2008-07-00 ·Pages 4629-41

Guan D, Altan-Bonnet N, Parrott AM, Arrigo CJ, Li Q, Khaleduzzaman M, Li H, Lee CG, Pe'ery T, Mathews MB

Abstract

Nuclear factor 90 (NF90) and its C-terminally extended isoform, NF110, have been isolated as DNA- and RNA-binding proteins together with the less-studied protein NF45. These complexes have been implicated in gene regulation, but little is known about their cellular roles and whether they are redundant or functionally distinct. We show that heterodimeric core complexes, NF90-NF45 and NF110-NF45, exist within larger complexes that are more labile and contain multiple NF90/110 isoforms and additional proteins. Depletion of the NF45 subunit by RNA interference is accompanied by a dramatic decrease in the levels of NF90 and NF110. Reciprocally, depletion of NF90 but not of NF110 greatly reduces the level of NF45. Coregulation of NF90 and NF45 is a posttranscriptional phenomenon, resulting from protein destabilization in the absence of partners. Depletion of NF90-NF45 complexes retards cell growth by inhibition of DNA synthesis. Giant multinucleated cells containing nuclei attached by constrictions accumulate when either NF45 or NF90, but not NF110, is depleted. This study identified NF45 as an unstable regulatory subunit of NF90-NF45 complexes and uncovered their critical role in normal cell division. Furthermore, the study revealed that NF90 is functionally distinct from NF110 and is more important for cell growth.

MeSH Terms
Cell Line Cell Nucleus/metabolism Giant Cells/metabolism HeLa Cells Humans Mitosis Nuclear Factor 45 Protein/genetics,metabolism Nuclear Factor 90 Proteins/genetics,metabolism Protein Processing, Post-Translational RNA Interference
Chemicals
ILF2 protein, human ILF3 protein, human Nuclear Factor 45 Protein Nuclear Factor 90 Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Guan Deyu
Departments of Biochemistry and Molecular Biology, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103-1709, USA.
Altan-Bonnet Nihal
Parrott Andrew M
Arrigo Cindy J
Li Quan
Khaleduzzaman Mohammed
Li Hong
Lee Chee-Gun
Pe'ery Tsafi
Mathews Michael B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.00120-08
Published
2008-07-00
Epub
2008-00-05
Pages
4629-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2447130
Subset
IM
Grants
NIGMS NIH HHS · F32 GM069139 · United States
NIAID NIH HHS · R01 AI034552 · United States
NIGMS NIH HHS · GM069139 · United States
NIAID NIH HHS · AI34552 · United States
External Links
PubMed source DOI: 10.1128/MCB.00120-08 PMC Full Text View journal details
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