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PMID: 18469805 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Extra centrosomes and/or chromosomes prolong mitosis in human cells.

Nature cell biology ·Vol. 10 ·No. 6 ·2008-06-00 ·Pages 748-51

Yang Z, Loncarek J, Khodjakov A, Rieder CL

Abstract

Using laser microsurgery and cell fusion we have explored how additional centrosomes and/or chromosomes influence the duration of mitosis in human cells. We found that doubling the chromosome number added approximately 10 min to a 20 min division, whereas doubling the number of centrosomes added approximately 30 min more. Extra centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the spindle assembly checkpoint. Thus mitosis can be prolonged by non-genetic means and extra chromosomes and centrosomes probably contribute to the elevated mitotic index seen in many tumours.

MeSH Terms
Animals Cell Cycle Cell Line Cell Nucleus/metabolism Centrosome/metabolism,ultrastructure Chromosomes/metabolism,ultrastructure Humans Kinetochores/ultrastructure Microscopy, Video Microtubules/chemistry Mitosis Models, Biological Spindle Apparatus Time Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang Zhenye
Division of Molecular Medicine, Wadsworth Center, N.Y.S. Department of Health, Empire State Plaza, Albany, New York 12201-0509, USA.
Loncarek Jadranka
Khodjakov Alexey
Rieder Conly L
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1476-4679
DOI
10.1038/ncb1738
Published
2008-06-00
Epub
2008-00-11
Pages
748-51
Language
English
Region
England
NLM ID
100890575
PMCID
PMC2430725
Subset
IM
Grants
NIGMS NIH HHS · GM40198 · United States
NIGMS NIH HHS · GM59363 · United States
NIGMS NIH HHS · R01 GM059363 · United States
NIGMS NIH HHS · R37 GM040198 · United States
NIGMS NIH HHS · R37 GM040198-24 · United States
NIGMS NIH HHS · R01 GM059363-10 · United States
External Links
PubMed source DOI: 10.1038/ncb1738 PMC Full Text View journal details
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