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PMID: 18477458 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Sensory signaling-dependent remodeling of olfactory cilia architecture in C. elegans.

Developmental cell ·Vol. 14 ·No. 5 ·2008-05-00 ·Pages 762-74

Mukhopadhyay S, Lu Y, Shaham S, Sengupta P

Abstract

Nonmotile primary cilia are sensory organelles composed of a microtubular axoneme and a surrounding membrane sheath that houses signaling molecules. Optimal cellular function requires the precise regulation of axoneme assembly, membrane biogenesis, and signaling protein targeting and localization via as yet poorly understood mechanisms. Here, we show that sensory signaling is required to maintain the architecture of the specialized AWB olfactory neuron cilia in C. elegans. Decreased sensory signaling results in alteration of axoneme length and expansion of a membraneous structure, thereby altering the topological distribution of a subset of ciliary transmembrane signaling molecules. Signaling-regulated alteration of ciliary structures can be bypassed by modulation of intracellular cGMP or calcium levels and requires kinesin-II-driven intraflagellar transport (IFT), as well as BBS- and RAB8-related proteins. Our results suggest that compensatory mechanisms in response to altered levels of sensory activity modulate AWB cilia architecture, revealing remarkable plasticity in the regulation of cilia structure.

MeSH Terms
Animals Bacteria Biological Transport Caenorhabditis elegans/cytology,embryology,metabolism,ultrastructure Caenorhabditis elegans Proteins/metabolism Calcium/metabolism Cell Membrane/metabolism Cilia/ultrastructure Cyclic GMP/metabolism Embryonic Development Ion Channels/metabolism Kinesins/metabolism Larva Mutation/genetics Olfactory Receptor Neurons/cytology,ultrastructure Phenotype Protein Binding Signal Transduction
Chemicals
Caenorhabditis elegans Proteins Ion Channels tax-2 protein, C elegans kinesin-II Kinesins Cyclic GMP Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Mukhopadhyay Saikat
Department of Biology and National Center for Behavioral Genomics, Brandeis University, Waltham, MA 02454, USA.
Lu Yun
Shaham Shai
Sengupta Piali
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1878-1551
DOI
10.1016/j.devcel.2008.03.002
Published
2008-05-00
Pages
762-74
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC2442577
Subset
IM
Grants
NIGMS NIH HHS · R37 GM056223 · United States
NIGMS NIH HHS · GM56223 · United States
NIGMS NIH HHS · R01 GM056223-11 · United States
NINDS NIH HHS · P30 NS045713-049001 · United States
NCRR NIH HHS · S10 RR016708-01 · United States
NINDS NIH HHS · P30 NS045713 · United States
NIGMS NIH HHS · R01 GM056223 · United States
NINDS NIH HHS · P30NS45713 · United States
NCRR NIH HHS · S10RR16708 · United States
Corrections
CommentIn
PMID: 18477443
External Links
PubMed source DOI: 10.1016/j.devcel.2008.03.002 PMC Full Text View journal details
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