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PMID: 1848706 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Epidermal growth factor (EGF) receptor T669 peptide kinase from 3T3-L1 cells is an EGF-stimulated "MAP" kinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 6 ·1991-03-15 ·Pages 2520-4

Takishima K, Griswold-Prenner I, Ingebritsen T, Rosner MR

Abstract

The epidermal growth factor (EGF) receptor is both an activator and a target of growth factor-stimulated kinases involved in cellular signaling. Threonine-669 (T669) of the EGF receptor is phosphorylated in response to a wide variety of growth-modulating agents. MAP kinase is similarly phosphorylated as well as stimulated by growth activators, including EGF. To determine whether a MAP-type kinase is responsible for T669 kinase activity in EGF-stimulated 3T3-L1 cells, we partially purified and characterized the T669 peptide kinase. The results indicate that a MAP kinase phosphorylates the T669 peptide and raise the possibility that this enzyme may participate in a feedback loop, being activated by the EGF receptor and in turn phosphorylating the receptor.

MeSH Terms
Animals Cell Line Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Epidermal Growth Factor/pharmacology ErbB Receptors/metabolism Kinetics Mice Microtubule-Associated Proteins/metabolism Phosphorylation Protein-Tyrosine Kinases/isolation & purification,metabolism Threonine
Chemicals
Microtubule-Associated Proteins Threonine Epidermal Growth Factor ErbB Receptors Protein-Tyrosine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takishima K
Ben May Institute, Chicago, IL.
Griswold-Prenner I
Ingebritsen T
Rosner M R
References (26)
26 references, click to expand
  1. Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
    Nature. 1988 Aug 25;334(6184):715-8 PMID: 2842685
  2. Tumor-promoting phorbol diesters cause the phosphorylation of epidermal growth factor receptors in normal human fibroblasts at threonine-654.
    Proc Natl Acad Sci U S A. 1985 Apr;82(7):1974-8 PMID: 2984676
  3. Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism.
    Nature. 1989 Jan 5;337(6202):78-81 PMID: 2562908
  4. Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
    Proc Natl Acad Sci U S A. 1987 Mar;84(6):1502-6 PMID: 2951732
  5. Cell lines and peripheral blood leukocytes derived from individuals with chronic myelogenous leukemia display virtually identical proteins phosphorylated on tyrosine residues.
    Proc Natl Acad Sci U S A. 1987 Jul;84(13):4408-12 PMID: 2440021
  6. Cloning and characterization of the myelin basic protein gene from mouse: one gene can encode both 14 kd and 18.5 kd MBPs by alternate use of exons.
    Cell. 1985 Aug;42(1):139-48 PMID: 2410136
  7. Alternative splicing accounts for the four forms of myelin basic protein.
    Cell. 1985 Dec;43(3 Pt 2):721-7 PMID: 2416470
  8. Protein kinase C phosphorylation of the EGF receptor at a threonine residue close to the cytoplasmic face of the plasma membrane.
    Nature. 1984 Oct 4-10;311(5985):480-3 PMID: 6090944
  9. An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control.
    Science. 1990 Jul 6;249(4964):64-7 PMID: 2164259
  10. Signal transduction by receptors with tyrosine kinase activity.
    Cell. 1990 Apr 20;61(2):203-12 PMID: 2158859
  11. Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.
    J Biol Chem. 1990 Jul 15;265(20):11487-94 PMID: 2142153
  12. Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
    Nature. 1990 Feb 15;343(6259):651-3 PMID: 2154696
  13. Association of protein phosphatase 2A with polyoma virus medium tumor antigen.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2521-5 PMID: 2157202
  14. Cloning and expression of a protein-tyrosine-phosphatase.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1501-5 PMID: 2154749
  15. Phospholipase C-gamma is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro.
    Cell. 1989 Jun 30;57(7):1109-22 PMID: 2472219
  16. EGF induces tyrosine phosphorylation of phospholipase C-II: a potential mechanism for EGF receptor signaling.
    Cell. 1989 Jun 30;57(7):1101-7 PMID: 2472218
  17. Mechanism of phosphorylation of the epidermal growth factor receptor at threonine 669.
    J Biol Chem. 1989 Jun 25;264(18):10828-35 PMID: 2543683
  18. Direct activation of the serine/threonine kinase activity of Raf-1 through tyrosine phosphorylation by the PDGF beta-receptor.
    Cell. 1989 Aug 25;58(4):649-57 PMID: 2475255
  19. Evidence that pp42, a major tyrosine kinase target protein, is a mitogen-activated serine/threonine protein kinase.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):6940-3 PMID: 2550926
  20. PDGF induction of tyrosine phosphorylation of GTPase activating protein.
    Nature. 1989 Dec 7;342(6250):711-4 PMID: 2480526
  21. The colony stimulating factor-1 receptor associates with and activates phosphatidylinositol-3 kinase.
    Nature. 1989 Dec 7;342(6250):699-702 PMID: 2556641
  22. Epidermal growth factor stimulates tyrosine phosphorylation of phospholipase C-II independently of receptor internalization and extracellular calcium.
    Proc Natl Acad Sci U S A. 1989 Mar;86(5):1568-72 PMID: 2466293
  23. Phosphorylation at threonine-654 is not required for negative regulation of the epidermal growth factor receptor by non-phorbol tumor promoters.
    Proc Natl Acad Sci U S A. 1989 Feb;86(3):812-6 PMID: 2492663
  24. Thapsigargin, a novel promoter, phosphorylates the epidermal growth factor receptor at threonine 669.
    Biochem Biophys Res Commun. 1988 Dec 15;157(2):740-6 PMID: 3202876
  25. Epidermal growth factor receptor threonine and serine residues phosphorylated in vivo.
    J Biol Chem. 1988 Sep 15;263(26):13152-8 PMID: 3138233
  26. Characterization of insulin-stimulated microtubule-associated protein kinase. Rapid isolation and stabilization of a novel serine/threonine kinase from 3T3-L1 cells.
    J Biol Chem. 1988 Sep 5;263(25):12721-7 PMID: 2842341
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.6.2520
Published
1991-03-15
Pages
2520-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51264
Subset
IM
Grants
NCI NIH HHS · CA35541 · United States
NIGMS NIH HHS · GM07197 · United States
NIGMS NIH HHS · GM38415 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.6.2520 PMC Full Text View journal details
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