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PMID: 1849072 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mbh 1: a novel gelsolin/severin-related protein which binds actin in vitro and exhibits nuclear localization in vivo.

The EMBO journal ·Vol. 10 ·No. 4 ·1991-04-00 ·Pages 757-66

Prendergast GC, Ziff EB

Abstract

We describe the characterization of a novel cDNA, mbh1 (myc basic motif homolog-1), which was found during a search for candidate factors which might interact with the c-Myc oncoprotein. Embedded within the amino acid sequence encoded by mbh1 is a region distantly related to the basic/helix-loop-helix (B/HLH) DNA-binding motif and a potential nuclear localization signal. Mbh1 encodes a polypeptide structurally similar to the actin-severing proteins gelsolin and severin. Translation of mbh1 RNA in rabbit reticulocyte extracts produces an approximately 45 kd protein capable of binding actin-coupled agarose beads in vitro in a Ca2(+)-dependent manner. Antiserum raised to a trpE/mbh1 bacterial fusion protein recognizes an approximately 45 kb protein in murine 3T3 fibroblasts, suggesting that the cDNA encodes the complete Mbh1 protein. Examination of Mbh1 localization in 3T3 fibroblasts by indirect immunofluorescence reveals a larger cell population showing diffuse staining, and a smaller population exhibiting a distinct nuclear stain. Western analysis corroborates this intracellular localization and indicates that total cellular levels and localization of Mbh1 are not affected by the cell growth state. The data suggest that Mbh1 may play a role in regulating cytoplasmic and/or nuclear architecture through potential interactions with actin.

MeSH Terms
Actins/metabolism Amino Acid Sequence Animals Base Sequence Blotting, Western Calcium-Binding Proteins/genetics Carrier Proteins/genetics,metabolism Cell Line Cell Nucleus/metabolism Cloning, Molecular DNA-Binding Proteins/genetics,metabolism Fungal Proteins/genetics Gelsolin Gene Library Genes, myc Mice Microfilament Proteins/genetics Molecular Sequence Data Nuclear Proteins Oligonucleotide Probes Polymerase Chain Reaction Protein Binding Protozoan Proteins Rabbits Reticulocytes/metabolism Sequence Homology, Nucleic Acid
Chemicals
Actins Calcium-Binding Proteins Carrier Proteins DNA-Binding Proteins Fungal Proteins Gelsolin Microfilament Proteins Nuclear Proteins Oligonucleotide Probes Protozoan Proteins severin protein, Dictyostelium Capg protein, mouse
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prendergast G C
Department of Biochemistry, New York University Medical Center, NY 10016.
Ziff E B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-04-00
Pages
757-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452713
Subset
IM
Databases
GENBANK
X53923, X53924, X53925, X54511, X56572, X56573, X56574, X57260, X57261, X57262
External Links
PubMed source PMC Full Text View journal details
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