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PMID: 1850038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Role of a viral membrane polypeptide in strand-specific initiation of poliovirus RNA synthesis.

Journal of virology ·Vol. 65 ·No. 5 ·1991-05-00 ·Pages 2647-54

Giachetti C, Semler BL

Abstract

A molecular genetic analysis has been combined with an in vitro biochemical approach to define the functional interactions required for nucleotidyl protein formation during poliovirus RNA synthesis. A site-directed lesion into the hydrophobic domain of a viral membrane protein produced a mutant virus that is defective in RNA synthesis at 39 degrees C. The phenotypic expression of this lesion affects initiation of RNA synthesis, in vitro uridylylation of the genome-linked protein (VPg), and the in vivo synthesis of plus-strand viral RNAs. Our results support a model that employs a viral membrane protein as carrier for VPg in the initiation of plus-strand RNA synthesis. Our data also suggest that a separate mechanism could be used in the initiation of minus-strand RNA synthesis, thereby providing a means for strand-specific regulation of picornavirus RNA replication.

MeSH Terms
Amino Acid Sequence DNA, Viral Gene Expression Regulation, Viral Kinetics Molecular Sequence Data Mutation Phenotype Poliovirus/genetics RNA, Viral/biosynthesis Temperature Viral Envelope Proteins/metabolism
Chemicals
DNA, Viral RNA, Viral Viral Envelope Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Giachetti C
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
Semler B L
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.5.2647-2654.1991
Published
1991-05-00
Pages
2647-54
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240623
Subset
IM
Grants
NIAID NIH HHS · R01 AI022693 · United States
NIAID NIH HHS · AI00721 · United States
NIAID NIH HHS · AI22693 · United States
Corrections
ErratumIn
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ErratumIn
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External Links
PubMed source DOI: 10.1128/JVI.65.5.2647-2654.1991 PMC Full Text View journal details
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