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PMID: 18504607 Published · ppublish English Journal Article

Context-dependent regulation of Hox protein functions by CK2 phosphorylation sites.

Development genes and evolution ·Vol. 218 ·No. 6 ·2008-06-00 ·Pages 321-32

Taghli-Lamallem O, Hsia C, Ronshaugen M, McGinnis W

Abstract

Variations in Hox protein sequences and functions have been proposed to contribute to evolutionary changes in appendage shape and number in crustaceans and insects. One model is that insect Hox proteins of the Ultrabithorax (UBX) ortholog class evolved increased abilities to repress Distal-less (Dll) transcription and appendage development in part through the loss of serine and threonine residues in casein kinase 2 (CK2) phosphorylation sites. To explore this possibility, we constructed and tested the appendage repression function of chimeric proteins with insertions of different CK2 consensus sites or phosphomimetics of CK2 sites in C-terminal regions of Drosophila melanogaster UBX. Our results indicate that CK2 sites C-terminal to the homeodomain can inhibit the appendage repression functions of UBX proteins, but only in the context of specific amino acid sequences. Our results, combined with previous findings on evolutionary changes in Hox protein, suggest how intra-protein regulatory changes can diversify Hox protein function, and thus animal morphology.

MeSH Terms
Amino Acid Sequence Animals Animals, Genetically Modified Binding Sites Body Patterning/genetics Casein Kinase II/metabolism Chimera/genetics Drosophila Proteins/genetics Drosophila melanogaster/embryology,genetics,metabolism Embryo, Nonmammalian Gene Expression Regulation, Developmental Homeodomain Proteins/chemistry,genetics,metabolism,physiology Molecular Sequence Data Phosphorylation Recombinant Fusion Proteins/genetics,metabolism Sequence Homology, Amino Acid Thorax/embryology Transcription Factors/genetics
Chemicals
Drosophila Proteins Homeodomain Proteins Recombinant Fusion Proteins Transcription Factors Ubx protein, Drosophila Casein Kinase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Taghli-Lamallem Ouarda
Neuroscience & Aging Research Center, The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
Hsia Cheryl
Ronshaugen Matthew
McGinnis William
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Article Info
Journal
Development genes and evolution
Abbr.
Dev Genes Evol
ISSN
0949-944X
DOI
10.1007/s00427-008-0224-1
Published
2008-06-00
Epub
2008-00-27
Pages
321-32
Language
English
Region
Germany
NLM ID
9613264
PMCID
PMC2443945
Subset
IM
Grants
NICHD NIH HHS · R37 HD028315 · United States
NICHD NIH HHS · R37 HD028315-17 · United States
External Links
PubMed source DOI: 10.1007/s00427-008-0224-1 PMC Full Text View journal details
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