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PMID: 1850833 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transient occurrence of extrachromosomal DNA of an Arabidopsis thaliana transposon-like element, Tat1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3618-22

Peleman J, Cottyn B, Van Camp W, Van Montagu M, Inzé D

Abstract

Analysis of 11 genomic clones containing the S-adenosylmethionine synthetase 1 gene (sam1) of Arabidopsis thaliana revealed the presence of a 431-base-pair (bp) insertion in the 3' end of sam1 in one of these clones. The inserted sequence, called Tat1, shows structural features of a transposon. It is flanked by a 5-bp duplication of the target site DNA and has 13-bp inverted repeats at its termini. Two highly homologous elements situated in a different genomic context were isolated from a genomic library. Genomic Southern analysis indicates that there are at least four copies of Tat1 present in the A. thaliana ecotype Columbia genome. Different hybridization patterns are observed with DNAs derived from different ecotypes of Arabidopsis thaliana, indicating that the element has moved since the divergence of these ecotypes. In two populations of A. thaliana, linear extrachromosomal Tat1-homologous DNA has been observed. The presented data are consistent with the hypothesis that Tat1 is an active transposable element.

Related Genes

Tat1 sam1

MeSH Terms

Base Sequence Blotting, Southern Cloning, Molecular DNA Transposable Elements Extrachromosomal Inheritance Genes, Plant Methionine Adenosyltransferase/genetics Molecular Sequence Data Plants/genetics RNA Probes Restriction Mapping

Chemicals

DNA Transposable Elements RNA Probes Methionine Adenosyltransferase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Peleman J

Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.

Cottyn B

Van Camp W

Van Montagu M

Inzé D

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.88.9.3618

Published

1991-05-01

Pages

3618-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC51503

Subset

Databases

GENBANK

M55077, M55144, M55145, M55146, M60366, M60367, M60368, M60369, M62384, M62385, M62386

External Links

PubMed source DOI: 10.1073/pnas.88.9.3618 PMC Full Text View journal details

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Transient occurrence of extrachromosomal DNA of an Arabidopsis thaliana transposon-like element, Tat1.

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