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PMID: 1852002 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Class II-antigen-negative patient and mutant B-cell lines represent at least three, and probably four, distinct genetic defects defined by complementation analysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 10 ·1991-05-15 ·Pages 4285-8

Bénichou B, Strominger JL

Abstract

Expression of class II major histocompatibility complex antigens in defective B-lymphoblastoid cell lines from patients with class II antigen deficiency and from in vitro mutants generated with the same phenotype was studied. By heterogenetic fusion experiments, at least three, and probably four, complementation groups were defined. Furthermore, clone 13 (a DR-, DP-, but DQ+ cell line) appeared to belong to the RJ2.2.5 complementation group, for which all other members are DR-, DP-, and also DQ-. Thus, it is hypothesized that the cell lines of this group lack the activity of a gene that can differentially regulate the DR/DP and the DQ promoters.

MeSH Terms
B-Lymphocytes/immunology Cell Line, Transformed Gene Expression Gene Expression Regulation Genes, MHC Class II Genetic Complementation Test HLA-DP Antigens/genetics HLA-DQ Antigens/genetics HLA-DR Antigens/genetics Herpesvirus 4, Human Histocompatibility Antigens Class II/deficiency,genetics Humans Mutagenesis Mutation Nucleic Acid Hybridization Phenotype Promoter Regions, Genetic
Chemicals
HLA-DP Antigens HLA-DQ Antigens HLA-DR Antigens Histocompatibility Antigens Class II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bénichou B
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Strominger J L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.10.4285
Published
1991-05-15
Pages
4285-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51643
Subset
IM
Grants
NCI NIH HHS · CA-47554 · United States
NIDDK NIH HHS · DK-30241 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.10.4285 PMC Full Text View journal details
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