Characterization of pig rotavirus RNA.

Pig rotavirus was purified from faeces. The RNA from this virus was resistant to pancreatic ribonuclease, indicating that it is double-stranded. When electrophoresed on polyacrylamide-agarose gels, pig rotavirus RNA migrated as 9 bands comprised of 11 or 12 RNA segments with a total mol. wt. of approx. 11 X 10(6). Co-electrophoresis experiments revealed that the RNAs from the pig virus and two isolates of the calf rotavirus were indistinguishable.