Home LiteratureArticle Details
PMID: 18533226 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural

Aging and dietary restriction effects on ubiquitination, sumoylation, and the proteasome in the heart.

Mechanisms of ageing and development ·Vol. 129 ·No. 9 ·2008-09-00 ·Pages 515-21

Li F, Zhang L, Craddock J, Bruce-Keller AJ, Dasuri K, Nguyen A, Keller JN

Abstract

Dietary restriction (DR), in the absence of malnutrition, is the only intervention known to reliably increase average and maximal lifespan in a variety of organisms including mammals. Because the effects of DR on the heart are poorly understood, in the present study we examined the effects of DR on the ubiquitin-proteasome pathway (UPP) in the heart. In these studies we observed that DR significantly reduced age-related impairments in proteasome-mediated protein degradation, and reduced age-related increases in ubiquitinated, oxidized, and sumoylated protein in the heart. Interestingly, DR did not significantly increase the expression of 20S proteasome subunits or the proteasome maturation factor (POMP-1). These data demonstrate for the first time the effects of aging and DR on proteasome biogenesis and sumoylation in the heart. Cumulatively, our data indicate that DR has many beneficial effects towards the UPP in the heart, and suggests that a preservation of the UPP may be a potential mechanism by which DR mediates beneficial effects on the cardiovascular system.

MeSH Terms
Aging/metabolism,physiology Animals Caloric Restriction Heart/physiology Male Myocardium/enzymology Myocytes, Cardiac/enzymology,metabolism,physiology Proteasome Endopeptidase Complex/metabolism,physiology Proteins/metabolism Rats Rats, Inbred BN Rats, Inbred F344 Signal Transduction/physiology Small Ubiquitin-Related Modifier Proteins/metabolism Ubiquitin/metabolism,physiology Ubiquitination/physiology
Chemicals
Proteins Small Ubiquitin-Related Modifier Proteins Ubiquitin Proteasome Endopeptidase Complex
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Li Feng
Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY, USA.
Zhang Le
Craddock Jeffrey
Bruce-Keller Annadora J
Dasuri Kalavathi
Nguyen AnhThao
Keller Jeffrey N
References (36)
36 references, click to expand
  1. Ump1p is required for proper maturation of the 20S proteasome and becomes its substrate upon completion of the assembly.
    Cell. 1998 Feb 20;92(4):489-99 PMID: 9491890
  2. Calorie restriction improves cardiovascular risk factors via reduction of mitochondrial reactive oxygen species in type II diabetic rats.
    J Pharmacol Exp Ther. 2007 Feb;320(2):535-43 PMID: 17068205
  3. Structure and functions of the 20S and 26S proteasomes.
    Annu Rev Biochem. 1996;65:801-47 PMID: 8811196
  4. Interplay between protein synthesis and degradation in the CNS: physiological and pathological implications.
    Trends Neurosci. 2007 Jan;30(1):31-6 PMID: 17126920
  5. Alternate-day fasting and chronic disease prevention: a review of human and animal trials.
    Am J Clin Nutr. 2007 Jul;86(1):7-13 PMID: 17616757
  6. Proteasome inhibition induces reversible impairments in protein synthesis.
    FASEB J. 2006 Jun;20(8):1055-63 PMID: 16770004
  7. Mammalian proteasome subpopulations with distinct molecular compositions and proteolytic activities.
    Mol Cell Proteomics. 2007 Nov;6(11):2021-31 PMID: 17660509
  8. SUMO--nonclassical ubiquitin.
    Annu Rev Cell Dev Biol. 2000;16:591-626 PMID: 11031248
  9. The bitter end: the ubiquitin-proteasome system and cardiac dysfunction.
    Circulation. 2007 Mar 20;115(11):1456-63 PMID: 17372187
  10. Age-dependent declines in proteasome activity in the heart.
    Arch Biochem Biophys. 2002 Jan 15;397(2):298-304 PMID: 11795886
  11. Metabolic stabilization of MAP kinase phosphatase-2 in senescence of human fibroblasts.
    Exp Cell Res. 2003 Nov 1;290(2):195-206 PMID: 14567979
  12. Identification and characterization of a mammalian protein interacting with 20S proteasome precursors.
    Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10348-53 PMID: 10973495
  13. Proteasome regulation of oxidative stress in aging and age-related diseases of the CNS.
    Antioxid Redox Signal. 2006 Jan-Feb;8(1-2):163-72 PMID: 16487050
  14. Influence of caloric restriction on aging immune system.
    J Nutr Health Aging. 2004;8(1):38-47 PMID: 14730366
  15. Autophagy and aging: the importance of maintaining "clean" cells.
    Autophagy. 2005 Oct-Dec;1(3):131-40 PMID: 16874025
  16. Aging, lipofuscin formation, and free radical-mediated inhibition of cellular proteolytic systems.
    Ageing Res Rev. 2003 Oct;2(4):383-405 PMID: 14522242
  17. Oxidative stress, aging and the proteasomal system.
    Biogerontology. 2000;1(1):31-40 PMID: 11707918
  18. Oxidative modification and inactivation of the proteasome during coronary occlusion/reperfusion.
    J Biol Chem. 2001 Aug 10;276(32):30057-63 PMID: 11375979
  19. Oxidative inactivation of the proteasome in Alzheimer's disease.
    Free Radic Res. 2007 Jun;41(6):673-80 PMID: 17516240
  20. Decreased levels of proteasome activity and proteasome expression in aging spinal cord.
    Neuroscience. 2000;98(1):149-56 PMID: 10858621
  21. Possible involvement of proteasome inhibition in aging: implications for oxidative stress.
    Mech Ageing Dev. 2000 Jan 24;113(1):61-70 PMID: 10708250
  22. Proteasome inhibition promotes regression of left ventricular hypertrophy.
    Am J Physiol Heart Circ Physiol. 2008 Feb;294(2):H645-50 PMID: 18032525
  23. Effects of aging and dietary restriction on ubiquitination, sumoylation, and the proteasome in the spleen.
    FEBS Lett. 2007 Nov 27;581(28):5543-7 PMID: 17991438
  24. Proteasome inhibitors shorten replicative life span and induce a senescent-like phenotype of human fibroblasts.
    J Cell Physiol. 2006 Jun;207(3):845-53 PMID: 16523493
  25. Synthesis, modifications, and turnover of proteins during aging.
    Exp Gerontol. 1996 Jan-Apr;31(1-2):33-47 PMID: 8706803
  26. Proteolysis, caloric restriction and aging.
    Mech Ageing Dev. 2001 May 31;122(7):595-615 PMID: 11322989
  27. Calorie restriction reduced blood pressure in obesity hypertensives by improvement of autonomic nerve activity and insulin sensitivity.
    J Cardiovasc Pharmacol. 2001 Oct;38 Suppl 1:S69-74 PMID: 11811363
  28. Caloric restriction and aging: an update.
    Exp Gerontol. 2000 May;35(3):299-305 PMID: 10832051
  29. 20S proteasome biogenesis.
    Biochimie. 2001 Mar-Apr;83(3-4):289-93 PMID: 11295488
  30. Beneficial effects of intermittent fasting and caloric restriction on the cardiovascular and cerebrovascular systems.
    J Nutr Biochem. 2005 Mar;16(3):129-37 PMID: 15741046
  31. Protein synthesis and degradation during starvation-induced cardiac atrophy in rabbits.
    Circ Res. 1987 Jun;60(6):933-41 PMID: 3594760
  32. Long-term calorie restriction is highly effective in reducing the risk for atherosclerosis in humans.
    Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6659-63 PMID: 15096581
  33. Binding of hydrophobic peptides to several non-catalytic sites promotes peptide hydrolysis by all active sites of 20 S proteasomes. Evidence for peptide-induced channel opening in the alpha-rings.
    J Biol Chem. 2002 Jun 21;277(25):22260-70 PMID: 11927581
  34. Aging and oxidative stress: modulation by dietary restriction.
    Free Radic Biol Med. 1996;21(5):651-68 PMID: 8891668
  35. Vitamin A deficiency leads to increased cell proliferation in olfactory epithelium of mature rats.
    J Neurobiol. 2003 Mar;54(4):539-54 PMID: 12555267
  36. Ubiquitin and ubiquitin-like proteins in protein regulation.
    Circ Res. 2007 May 11;100(9):1276-91 PMID: 17495234
Article Info
Journal
Mechanisms of ageing and development
Abbr.
Mech Ageing Dev
ISSN
0047-6374
DOI
10.1016/j.mad.2008.04.007
Published
2008-09-00
Epub
2008-00-30
Pages
515-21
Language
English
Region
Ireland
NLM ID
0347227
PMCID
PMC2546525
Subset
IM
Grants
NIA NIH HHS · R01 AG029885-02 · United States
NIA NIH HHS · AG0257701 · United States
NIA NIH HHS · R01 AG025771-02 · United States
NIA NIH HHS · R01 AG025771 · United States
NIA NIH HHS · R01 AG029885 · United States
NIA NIH HHS · AG029885 · United States
External Links
PubMed source DOI: 10.1016/j.mad.2008.04.007 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2