Origin of polyoma virus-associated endonuclease.

Journal of virology ·Vol. 20 ·No. 1 ·1976-10-00 ·Pages 9-13

Rouget P, Parodi A, Blangy D, Cuzin F

Abstract

Polyoma virus particles purified from infected cells, but not from the culture medium, exhibited an endonuclease activity distinct from the serum contaminant recently described. This endonuclease cleaved form I polyoma DNA once only per molecule, at one of three possible sites corresponding to the known adenosine-ribosylthymine-rich regions of the molecule.

MeSH Terms

Cell Line DNA, Circular/metabolism DNA, Viral/metabolism Deoxyribonucleases/metabolism Endonucleases/metabolism Polyomavirus/enzymology

Chemicals

DNA, Circular DNA, Viral Deoxyribonucleases Endonucleases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Rouget P

Parodi A

Blangy D

Cuzin F

References (10)

10 references, click to expand

Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials.
Virology. 1962 Jan;16:41-51 PMID: 13926482
Purification of polyoma virus.
Virology. 1963 Feb;19:158-68 PMID: 14001211
Denaturation of polyoma DNA by phage T4 gene 32 protein.
Nature. 1975 May 15;255(5505):249-52 PMID: 167284
Location of the T4 gene 32 protein binding site on polyoma virus DNA.
FEBS Lett. 1975 Sep 15;57(2):126-9 PMID: 170135
Characterization of the single-strand-specific nuclease S1 activity on double-stranded supercoiled polyoma DNA.
Eur J Biochem. 1974 Apr 16;43(3):591-600 PMID: 4364862
Endonucleolytic cleavage of polyoma virus DNA: general properties and site specificity of the virion-associated endonuclease.
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:247-54 PMID: 239808
Selective extraction of polyoma DNA from infected mouse cell cultures.
J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
Cleavage of Simian virus 40 DNA at a unique site by a bacterial restriction enzyme.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3365-9 PMID: 4343967
Location of the T4 gene 32 protein-binding site on polyoma virus DNA.
Biochem Biophys Res Commun. 1974 Apr 23;57(4):1074-9 PMID: 4364559
Origin of the polyoma virus-associated endonuclease.
J Virol. 1975 Jan;17(1):127-31 PMID: 173877

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.20.1.9-13.1976

Published

1976-10-00

Pages

9-13

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC354959

Subset

External Links

PubMed source DOI: 10.1128/JVI.20.1.9-13.1976 PMC Full Text View journal details

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Origin of polyoma virus-associated endonuclease.

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