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PMID: 1855973 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Borreliacidal activity of sera from hamsters infected with the Lyme disease spirochete.

Infection and immunity ·Vol. 59 ·No. 8 ·1991-08-00 ·Pages 2522-8

Lovrich SD, Callister SM, Schmitz JL, Alder JD, Schell RF

Abstract

An in vitro borreliacidal assay that accurately reflects the levels of protective antibody determined by passive transfer of immunity studies was developed. Borreliacidal antibody in sera obtained from normal hamsters infected with Borrelia burgdorferi was readily detected. When immune serum containing complement was incubated with B. burgdorferi organisms, spirochetes were killed within 2 h. Treating immune serum with anti-hamster immunoglobulin G abrogated the borreliacidal activity. Killing of B. burgdorferi in serum was detected 1 week after infection; it peaked at week 3 and gradually declined. Relatively high levels of borreliacidal antibody were found, especially in week 3 immune serum, which could be diluted 1,280-fold. The decrease in borreliacidal antibody after infection may account for occurrences of reinfection and the remitting course of Lyme disease.

MeSH Terms
Animals Antibodies, Bacterial/blood,immunology Antibody Formation/immunology Borrelia burgdorferi Borrelia burgdorferi Group/growth & development,immunology,ultrastructure Cricetinae Immunization, Passive Kinetics Lyme Disease/immunology Methods Microscopy, Electron
Chemicals
Antibodies, Bacterial
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lovrich S D
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison 53706.
Callister S M
Schmitz J L
Alder J D
Schell R F
References (25)
25 references, click to expand
  1. Histopathology of Lyme arthritis in LSH hamsters.
    Am J Pathol. 1989 May;134(5):1113-23 PMID: 2719078
  2. Preparation of experimental animal tissue for SEM.
    Scan Electron Microsc. 1980;(Pt 2):1-19 PMID: 6999593
  3. Antiborrelial activity of serum from rats injected with the Lyme disease spirochete.
    J Infect Dis. 1991 Mar;163(3):656-9 PMID: 1995740
  4. Lyme borreliosis: host responses to Borrelia burgdorferi.
    Microbiol Rev. 1991 Mar;55(1):21-34 PMID: 2030671
  5. Characterization of the protective antibody response to Borrelia burgdorferi in experimentally infected LSH hamsters.
    Infect Immun. 1991 Jun;59(6):1916-21 PMID: 2037352
  6. Protection of mice against the Lyme disease agent by immunizing with recombinant OspA.
    Science. 1990 Oct 26;250(4980):553-6 PMID: 2237407
  7. Role of immunoglobulin G in killing of Borrelia burgdorferi by the classical complement pathway.
    Infect Immun. 1988 Feb;56(2):314-21 PMID: 3276623
  8. Experimental Lyme arthritis in rats infected with Borrelia burgdorferi.
    J Infect Dis. 1988 Apr;157(4):842-6 PMID: 3258003
  9. Experimental infection of the hamster with Borrelia burgdorferi.
    Ann N Y Acad Sci. 1988;539:258-63 PMID: 3190098
  10. Biology of Borrelia species.
    Microbiol Rev. 1986 Dec;50(4):381-400 PMID: 3540570
  11. Infection of Syrian hamsters with Lyme disease spirochetes.
    J Clin Microbiol. 1984 Dec;20(6):1099-101 PMID: 6520220
  12. Susceptibility of the hispid cotton rat (Sigmodon hispidus) to the Lyme disease spirochete (Borrelia burgdorferi).
    Am J Trop Med Hyg. 1987 Nov;37(3):624-8 PMID: 3688316
  13. Vaccination of hamsters against experimental infection with Borrelia burgdorferi.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Dec;263(1-2):45-8 PMID: 3577492
  14. Reservoir competence of white-footed mice for Lyme disease spirochetes.
    Am J Trop Med Hyg. 1987 Jan;36(1):92-6 PMID: 3812887
  15. Active immunization of hamsters against experimental infection with Borrelia burgdorferi.
    Infect Immun. 1986 Dec;54(3):897-8 PMID: 3781630
  16. Passive immunization of hamsters against experimental infection with the Lyme disease spirochete.
    Infect Immun. 1986 Sep;53(3):713-4 PMID: 3744563
  17. Reinfection in erythema migrans disease.
    Infection. 1986 Jan-Feb;14(1):32-5 PMID: 3957434
  18. The histopathology of experimentally infected hamsters with the Lyme disease spirochete, Borrelia burgdorferi.
    Proc Soc Exp Biol Med. 1986 Feb;181(2):263-9 PMID: 3945634
  19. Sequestration of antibody to Borrelia burgdorferi in immune complexes in seronegative Lyme disease.
    Lancet. 1990 Feb 10;335(8685):312-5 PMID: 1967770
  20. Effects of bovine serum albumin on the ability of Barbour-Stoenner-Kelly medium to detect Borrelia burgdorferi.
    J Clin Microbiol. 1990 Feb;28(2):363-5 PMID: 2179264
  21. Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice.
    Proc Natl Acad Sci U S A. 1990 May;87(10):3768-72 PMID: 2339119
  22. Cross-antigenicity between the major surface proteins (ospA and ospB) and other proteins of Borrelia burgdorferi.
    J Immunol. 1990 Jan 1;144(1):284-9 PMID: 2295795
  23. Passive immunization prevents induction of Lyme arthritis in LSH hamsters.
    Infect Immun. 1990 Jan;58(1):144-8 PMID: 2294046
  24. The spirochetal etiology of Lyme disease.
    N Engl J Med. 1983 Mar 31;308(13):733-40 PMID: 6828118
  25. Induction of lyme arthritis in LSH hamsters.
    Infect Immun. 1988 Sep;56(9):2336-42 PMID: 3410540
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.8.2522-2528.1991
Published
1991-08-00
Pages
2522-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258050
Subset
IM
Grants
NIAID NIH HHS · AI-22199 · United States
External Links
PubMed source DOI: 10.1128/iai.59.8.2522-2528.1991 PMC Full Text View journal details
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