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PMID: 1856180 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cloning and characterization of a glutamine transport operon of Bacillus stearothermophilus NUB36: effect of temperature on regulation of transcription.

Journal of bacteriology ·Vol. 173 ·No. 15 ·1991-08-00 ·Pages 4877-88

Wu L, Welker NE

Abstract

We cloned and sequenced a fragment of the Bacillus stearothermophilus NUB36 chromosome that contains two open reading frames (ORFs) whose products were detected only in cells of cultures grown in complex medium at high temperature. The nucleotide sequence of the two ORFs exhibited significant identity to the sequence of the glnQ and glnH loci of the glutamine transport system in enteric bacteria. In addition, growth response to glutamine, sensitivity to the toxic glutamine analog gamma-L-glutamylhydrazide, and glutamine transport assays with parental strain NUB3621 and mutant strain NUB36500, in which the ORF1 coding segment in the chromosome was interrupted with the cat gene, demonstrated that glnQ and glnH encode proteins that are active in the glutamine transport system in B. stearothermophilus. The inferred promoter for the glnQH operon exhibited a low homology to the -35 and -10 regions of the consensus promoter sequences of Bacillus subtilis and Escherichia coli genes. In addition, the inferred promoter for the glnQH operon also exhibited a low homology with the consensus promoter sequence deduced from the sequences of the promoters of nine different genes from B. stearothermophilus. Transcription of the glnQH operon was activated in a nitrogen-rich medium at high temperature and inhibited under the same conditions at low temperature. Transcription of the glnQH operon was partially activated in a nitrogen-poor medium at low temperature. The region upstream from glnQ contains sequences that have a low homology with the nitrogen regulator I-binding sequences and the nitrogen-regulated promoters of enteric bacteria. The effect of temperature on the regulation of the glnQH operon is discussed.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,isolation & purification Base Sequence Biological Transport, Active Cloning, Molecular Gene Expression Regulation, Bacterial Geobacillus stearothermophilus/genetics,growth & development,metabolism Glutamine/genetics,metabolism Hot Temperature Molecular Sequence Data Open Reading Frames Operon Protein Biosynthesis Transcription, Genetic
Chemicals
Bacterial Proteins Glutamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wu L
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208.
Welker N E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.15.4877-4888.1991
Published
1991-08-00
Pages
4877-88
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208167
Subset
IM
Databases
GENBANK
M61017, M74478, M74479, M74480, M74481, M74482, M74483, M74484, S45931, S45969
External Links
PubMed source DOI: 10.1128/jb.173.15.4877-4888.1991 PMC Full Text View journal details
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