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PMID: 18593557 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Hair follicle stem cells are specified and function in early skin morphogenesis.

Cell stem cell ·Vol. 3 ·No. 1 ·2008-07-03 ·Pages 33-43

Nowak JA, Polak L, Pasolli HA, Fuchs E

Abstract

In adult skin, epithelial hair follicle stem cells (SCs) reside in a quiescent niche and are essential for cyclic bouts of hair growth. Niche architecture becomes pronounced postnatally at the start of the first hair cycle. Whether SCs exist or function earlier is unknown. Here we show that slow-cycling cells appear early in skin development, express SC markers, and later give rise to the adult SC population. To test whether these early slow-cycling cells function as SCs, we use Sox9-Cre for genetic marking and K14-Cre to embryonically ablate Sox9, an essential adult SC gene. We find that the progeny of Sox9-expressing cells contribute to all skin epithelial lineages and Sox9 is required for SC specification. In the absence of early SCs, hair follicle and sebaceous gland morphogenesis is blocked, and epidermal wound repair is compromised. These findings establish the existence of early hair follicle SCs and reveal their physiological importance in tissue morphogenesis.

MeSH Terms
Adult Animals Biomarkers Cell Cycle Epithelial Cells/cytology,physiology Hair Follicle/cytology,physiology High Mobility Group Proteins/physiology Humans Integrases/metabolism Mice Morphogenesis SOX9 Transcription Factor Skin/cytology,growth & development Skin Physiological Phenomena Stem Cell Transplantation Stem Cells/cytology,physiology Transcription Factors/physiology Wound Healing
Chemicals
Biomarkers High Mobility Group Proteins SOX9 Transcription Factor SOX9 protein, human Sox9 protein, mouse Transcription Factors Cre recombinase Integrases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nowak Jonathan A
Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065, USA.
Polak Lisa
Pasolli H Amalia
Fuchs Elaine
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Article Info
Journal
Cell stem cell
Abbr.
Cell Stem Cell
ISSN
1875-9777
DOI
10.1016/j.stem.2008.05.009
Published
2008-07-03
Pages
33-43
Language
English
Region
United States
NLM ID
101311472
PMCID
PMC2877596
Subset
IM
Grants
NIAMS NIH HHS · R01 AR031737-21 · United States
NIAMS NIH HHS · R01 AR050452-05 · United States
NIAMS NIH HHS · R01 AR031737-24 · United States
NIAMS NIH HHS · R01 AR031737-20 · United States
NIAMS NIH HHS · R01 AR031737-21S1 · United States
Howard Hughes Medical Institute · United States
NIAMS NIH HHS · R01 AR031737-25 · United States
NIAMS NIH HHS · R01 AR031737-27S1 · United States
NIAMS NIH HHS · R01 AR031737-22 · United States
NIAMS NIH HHS · R01 AR031737 · United States
NIAMS NIH HHS · R01 AR031737-27 · United States
NIAMS NIH HHS · R01 AR050452 · United States
NIAMS NIH HHS · R01 AR031737-23 · United States
NIAMS NIH HHS · R01 AR031737-26 · United States
NCI NIH HHS · T32 CA009673 · United States
NIAMS NIH HHS · R01 AR031737-19 · United States
NIGMS NIH HHS · T32 GM007739 · United States
Corrections
CommentIn
PMID: 18593550
External Links
PubMed source DOI: 10.1016/j.stem.2008.05.009 PMC Full Text View journal details
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