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PMID: 1860823 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The Actinobacillus pleuropneumoniae hemolysin determinant: unlinked appCA and appBD loci flanked by pseudogenes.

Journal of bacteriology ·Vol. 173 ·No. 16 ·1991-08-00 ·Pages 5151-8

Chang YF, Young R, Struck DK

Abstract

The appBD genes encoding the secretion functions for the 110-kDa RTX hemolysin of Actinobacillus pleuropneumoniae have been cloned and sequenced. Unlike analogous genes from other RTX determinants, the appBD genes do not lie immediately downstream from the hemolysin structural gene, appA. Although isolated from a diverse group of gram-negative organisms, the appBD genes and the characterized RTX BD genes from other organisms all exhibit a high degree of homology at both the DNA and predicted amino acid sequence levels. Analysis of the DNA sequences 3' to appA and 5' to appB suggests that these regions harbor remnant RTX B and A pseudogenes, respectively. Although the appA gene is most similar to the lktA gene from Pasteurella haemolytica (Y. F. Chang, R. Young, and D. K. Struck, DNA 8:635-647, 1989), the RTX A pseudogene upstream from appB most closely resembles the hlyB gene from Escherichia coli, suggesting that the appCA and appBD operons were derived from different ancestral RTX determinants.

Related Genes
appB appD hlyB iktA
MeSH Terms
Actinobacillus/genetics Amino Acid Sequence Base Sequence Blotting, Southern Blotting, Western Carrier Proteins/genetics Escherichia coli/genetics Hemolysin Proteins/genetics Molecular Sequence Data Operon/genetics Pasteurella/genetics Pseudogenes/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Carrier Proteins Hemolysin Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chang Y F
Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14851.
Young R
Struck D K
References (30)
30 references, click to expand
  1. Structure and function of haemolysin B,P-glycoprotein and other members of a novel family of membrane translocators.
    Mol Microbiol. 1990 Jun;4(6):873-80 PMID: 1977073
  2. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  3. A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium.
    J Cell Sci Suppl. 1989;11:45-57 PMID: 2693460
  4. Genetical and functional organisation of the Escherichia coli haemolysin determinant 2001.
    Mol Gen Genet. 1985;201(2):282-8 PMID: 3003531
  5. DNA sequence of the Pasteurella haemolytica leukotoxin gene cluster.
    DNA. 1989 Jan-Feb;8(1):15-28 PMID: 2707120
  6. Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1.
    Infect Immun. 1987 Sep;55(9):1987-96 PMID: 3040588
  7. Comparison of the haemolysin secretion protein HlyB from Proteus vulgaris and Escherichia coli; site-directed mutagenesis causing impairment of export function.
    Mol Gen Genet. 1988 Aug;213(2-3):551-5 PMID: 3054490
  8. Regulation of expression of the Pasteurella haemolytica leukotoxin determinant.
    J Bacteriol. 1989 Nov;171(11):5955-62 PMID: 2478522
  9. Transcriptional organization of the Escherichia coli hemolysin genes.
    J Bacteriol. 1988 Apr;170(4):1622-30 PMID: 2450867
  10. Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.
    DNA. 1989 Nov;8(9):635-47 PMID: 2693022
  11. Immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in Actinobacillus pleuropneumoniae, Actinobacillus suis, Pasteurella haemolytica, and Escherichia coli.
    Infect Immun. 1989 Oct;57(10):3210-3 PMID: 2674017
  12. Secretion of the Pasteurella leukotoxin by Escherichia coli.
    FEMS Microbiol Lett. 1989 Jul 15;51(1):169-73 PMID: 2673918
  13. Analysis of the Actinobacillus actinomycetemcomitans leukotoxin gene. Delineation of unique features and comparison to homologous toxins.
    J Biol Chem. 1989 Sep 15;264(26):15451-6 PMID: 2670940
  14. Cloning, nucleotide sequence, and characterization of genes encoding the secretion function of the Pasteurella haemolytica leukotoxin determinant.
    J Bacteriol. 1989 Feb;171(2):916-28 PMID: 2914876
  15. The secreted hemolysins of Proteus mirabilis, Proteus vulgaris, and Morganella morganii are genetically related to each other and to the alpha-hemolysin of Escherichia coli.
    J Bacteriol. 1987 Apr;169(4):1509-15 PMID: 3549692
  16. A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria.
    Nature. 1986 Oct 2-8;323(6087):448-50 PMID: 3762694
  17. Characterisation of HlyC and mechanism of activation and secretion of haemolysin from E. coli 2001.
    FEBS Lett. 1985 Aug 5;187(2):339-44 PMID: 3894051
  18. Nucleotide sequence of an Escherichia coli chromosomal hemolysin.
    J Bacteriol. 1985 Jul;163(1):94-105 PMID: 3891743
  19. Nucleotide sequence of the leukotoxin gene from Actinobacillus actinomycetemcomitans: homology to the alpha-hemolysin/leukotoxin gene family.
    Infect Immun. 1990 Apr;58(4):920-9 PMID: 2318535
  20. Secretion and expression of the Pasteurella haemolytica Leukotoxin.
    J Bacteriol. 1990 May;172(5):2343-50 PMID: 2185213
  21. Characterization of monoclonal antibodies against the Escherichia coli hemolysin.
    Infect Immun. 1990 Mar;58(3):822-7 PMID: 1689701
  22. TolC, an Escherichia coli outer membrane protein required for hemolysin secretion.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4776-80 PMID: 2112747
  23. Isolation of the Actinobacillus pleuropneumoniae haemolysin gene and the activation and secretion of the prohaemolysin by the HlyC, HlyB and HlyD proteins of Escherichia coli.
    Mol Microbiol. 1990 Jan;4(1):123-8 PMID: 2181233
  24. Sequence of the lktB gene from Actinobacillus actinomycetemcomitans.
    Nucleic Acids Res. 1990 Sep 11;18(17):5291 PMID: 2402457
  25. Nonreciprocal complementation of the hlyC and lktC genes of the Escherichia coli hemolysin and Pasteurella haemolytica leukotoxin determinants.
    Infect Immun. 1990 Mar;58(3):828-32 PMID: 2307519
  26. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  27. Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):789-800 PMID: 6364042
  28. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  29. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
    Anal Biochem. 1984 Apr;138(1):141-3 PMID: 6731838
  30. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.16.5151-5158.1991
Published
1991-08-00
Pages
5151-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208207
Subset
IM
Grants
NIGMS NIH HHS · R01 GM027099 · United States
Databases
GENBANK
M64121, M64122, M64123, M64124, M64125, M64126, M64127, M64128, M64129, M65808
External Links
PubMed source DOI: 10.1128/jb.173.16.5151-5158.1991 PMC Full Text View journal details
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