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PMID: 1861980 Published · ppublish English Journal Article

K-tuple frequency in the human genome and polymerase chain reaction.

Nucleic acids research ·Vol. 19 ·No. 14 ·1991-07-25 ·Pages 3887-91

Griffais R, André PM, Thibon M

Abstract

The frequency occurrences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer programs based on these results was written to choose primers to amplify DNA target sequences, either of human genes or of human infectious agents. The software also gave nested primer sequences which were used to synthesize non radioactive probes by PCR. We applied these two methods, primer selection and non radioactive probes, to easily and quickly set up very efficient PCR sets to work in the human genome context.

MeSH Terms
Algorithms Animals Base Sequence Cell Line DNA DNA Probes Databases, Factual Genome, Human Humans Molecular Sequence Data Polymerase Chain Reaction Software
Chemicals
DNA Probes DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Griffais R
Laboratoire des Chlamydiales et des Rickettsiales, Institut Pasteur, Paris, France.
André P M
Thibon M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.14.3887
Published
1991-07-25
Pages
3887-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328479
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.14.3887 PMC Full Text View journal details
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