Home LiteratureArticle Details
PMID: 18653319 Published · ppublish English Comparative Study Journal Article Review

Comparative genomics of the mycobacteriophages: insights into bacteriophage evolution.

Research in microbiology ·Vol. 159 ·No. 5 ·2008-06-00 ·Pages 332-9

Hatfull GF, Cresawn SG, Hendrix RW

Abstract

The recognition of the vast numbers of bacteriophages in the biosphere has prompted a renewal of interest in understanding their morphological and genetic diversity, and elucidating the evolutionary mechanisms that give rise to them. We have approached these questions by isolating and characterizing a collection of mycobacteriophages that infect a common bacterial host, Mycobacterium smegmatis. Comparative genomic analysis of 50 mycobacteriophages shows that they are highly diverse, although not uniformly so, that they are pervasively mosaic with a multitude of single gene modules, and that this mosaicism is generated through illegitimate recombination.

MeSH Terms
Amino Acid Sequence Evolution, Molecular Genome, Viral Molecular Sequence Data Mosaicism Mycobacteriophages/genetics Mycobacterium smegmatis/ultrastructure,virology Recombination, Genetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hatfull Graham F
Department of Biological Sciences and Pittsburgh Bacteriophage Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA. [email protected]
Cresawn Steven G
Hendrix Roger W
References (36)
36 references, click to expand
  1. Origins of highly mosaic mycobacteriophage genomes.
    Cell. 2003 Apr 18;113(2):171-82 PMID: 12705866
  2. The origins and ongoing evolution of viruses.
    Trends Microbiol. 2000 Nov;8(11):504-8 PMID: 11121760
  3. Expression of Mycobacteriophage Ms6 lysis genes is driven by two sigma(70)-like promoters and is dependent on a transcription termination signal present in the leader RNA.
    J Bacteriol. 2002 Jun;184(11):3034-43 PMID: 12003945
  4. Control of phage Bxb1 excision by a novel recombination directionality factor.
    PLoS Biol. 2006 Jun;4(6):e186 PMID: 16719562
  5. The complete genomes and proteomes of 27 Staphylococcus aureus bacteriophages.
    Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5174-9 PMID: 15788529
  6. State of the art: typing Mycobacterium tuberculosis.
    J Hosp Infect. 1995 Mar;29(3):169-76 PMID: 7615934
  7. Comments on the arrangement of the morphogenetic genes of bacteriophage lambda.
    J Mol Biol. 1974 Nov 25;90(1):20-5 PMID: 4453012
  8. Recombineering in Mycobacterium tuberculosis.
    Nat Methods. 2007 Feb;4(2):147-52 PMID: 17179933
  9. Molecular genetics of bacteriophage P22.
    Microbiol Rev. 1978 Jun;42(2):385-413 PMID: 353481
  10. The continuing challenges of leprosy.
    Clin Microbiol Rev. 2006 Apr;19(2):338-81 PMID: 16614253
  11. Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene.
    Mol Microbiol. 2003 Oct;50(2):463-73 PMID: 14617171
  12. DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics.
    Mol Microbiol. 1993 Feb;7(3):395-405 PMID: 8459766
  13. Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology.
    BMC Microbiol. 2006 Mar 06;6:23 PMID: 16519816
  14. Introduction of foreign DNA into mycobacteria using a shuttle phasmid.
    Nature. 1987 Jun 11-17;327(6122):532-5 PMID: 3473289
  15. Bacteriophage genomics.
    Curr Opin Microbiol. 2003 Oct;6(5):506-11 PMID: 14572544
  16. 5500 Phages examined in the electron microscope.
    Arch Virol. 2007 Feb;152(2):227-43 PMID: 17051420
  17. Comparative genomic analysis of 18 Pseudomonas aeruginosa bacteriophages.
    J Bacteriol. 2006 Feb;188(3):1184-7 PMID: 16428425
  18. Synonymous codon usage analysis of the mycobacteriophage Bxz1 and its plating bacteria M. smegmatis: identification of highly and lowly expressed genes of Bxz1 and the possible function of its tRNA species.
    J Biochem Mol Biol. 2004 Jul 31;37(4):487-92 PMID: 15469738
  19. The mouse foot-pad technique for cultivation of Mycobacterium leprae.
    Lepr Rev. 2006 Mar;77(1):5-24 PMID: 16715686
  20. CRISPR--a widespread system that provides acquired resistance against phages in bacteria and archaea.
    Nat Rev Microbiol. 2008 Mar;6(3):181-6 PMID: 18157154
  21. CRISPR provides acquired resistance against viruses in prokaryotes.
    Science. 2007 Mar 23;315(5819):1709-12 PMID: 17379808
  22. Leprosy and tuberculosis: an insight-review.
    Crit Rev Microbiol. 2007;33(1):15-66 PMID: 17453929
  23. Induction of bacteriophage from members of the Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum serocomplex.
    J Gen Microbiol. 1984 Aug;130(8):2059-66 PMID: 6470677
  24. Bacteriophage active against virulent Mycobacterium tuberculosis. I. Isolation and activity.
    Am J Public Health Nations Health. 1954 Oct;44(10):1326-33 PMID: 13197609
  25. Comparative molecular biology of lambdoid phages.
    Annu Rev Microbiol. 1994;48:193-222 PMID: 7826005
  26. Genome structure of mycobacteriophage D29: implications for phage evolution.
    J Mol Biol. 1998 May 29;279(1):143-64 PMID: 9636706
  27. Nucleotide sequence of coliphage HK620 and the evolution of lambdoid phages.
    J Mol Biol. 2001 Aug 24;311(4):657-79 PMID: 11518522
  28. Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages.
    J Mol Biol. 2000 May 26;299(1):27-51 PMID: 10860721
  29. Exploring the mycobacteriophage metaproteome: phage genomics as an educational platform.
    PLoS Genet. 2006 Jun;2(6):e92 PMID: 16789831
  30. Control of directionality in integrase-mediated recombination: examination of recombination directionality factors (RDFs) including Xis and Cox proteins.
    Nucleic Acids Res. 2001 Jun 1;29(11):2205-16 PMID: 11376138
  31. Structure, function, and biogenesis of the cell wall of Mycobacterium tuberculosis.
    Tuberculosis (Edinb). 2003;83(1-3):91-7 PMID: 12758196
  32. Genomic characterization of mycobacteriophage Giles: evidence for phage acquisition of host DNA by illegitimate recombination.
    J Bacteriol. 2008 Mar;190(6):2172-82 PMID: 18178732
  33. Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria.
    Microbiology (Reading). 2007 Aug;153(Pt 8):2711-2723 PMID: 17660435
  34. Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage.
    Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2192-7 PMID: 10051617
  35. Comparative phage genomics and the evolution of Siphoviridae: insights from dairy phages.
    Mol Microbiol. 2001 Jan;39(2):213-22 PMID: 11136444
  36. Efficient point mutagenesis in mycobacteria using single-stranded DNA recombineering: characterization of antimycobacterial drug targets.
    Mol Microbiol. 2008 Mar;67(5):1094-107 PMID: 18221264
Article Info
Journal
Research in microbiology
Abbr.
Res Microbiol
ISSN
0923-2508
DOI
10.1016/j.resmic.2008.04.008
Email
[email protected]
Published
2008-06-00
Epub
2008-00-07
Pages
332-9
Language
English
Region
France
NLM ID
8907468
PMCID
PMC2632949
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051975 · United States
NIGMS NIH HHS · R01 GM051975-08S1 · United States
External Links
PubMed source DOI: 10.1016/j.resmic.2008.04.008 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2