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PMID: 1865879 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation.

Molecular & general genetics : MGG ·Vol. 227 ·No. 3 ·1991-07-00 ·Pages 417-23

Fleischmann M, Clark MW, Forrester W, Wickens M, Nishimoto T, Aebi M

Abstract

Mutations in the PRP20 gene of yeast show a pleiotropic phenotype, in which both mRNA metabolism and nuclear structure are affected. srm1 mutants, defective in the same gene, influence the signal transduction pathway for the pheromone response. The yeast PRP20/SRM1 protein is highly homologous to the RCC1 protein of man, hamster and frog. In mammalian cells, this protein is a negative regulator for initiation of chromosome condensation. We report the analysis of two, independently isolated, recessive temperature-sensitive prp20 mutants. They have identical G to A transitions, leading to the alteration of a highly conserved glycine residue to glutamic acid. By immunofluorescence microscopy the PRP20 protein was localized in the nucleus. Expression of the RCC1 protein can complement the temperature-sensitive phenotype of prp20 mutants, demonstrating the functional similarity of the yeast and mammalian proteins.

Related Genes

PRP20

MeSH Terms

Alleles Blotting, Western Cell Cycle Proteins Chromosomes, Fungal Chromosomes, Human DNA-Binding Proteins/genetics Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Genes, Fungal Genetic Complementation Test Guanine Nucleotide Exchange Factors Humans Mutation Nuclear Proteins/genetics Phenotype Plasmids Precipitin Tests Saccharomyces cerevisiae/genetics Temperature

Chemicals

Cell Cycle Proteins DNA-Binding Proteins Guanine Nucleotide Exchange Factors Nuclear Proteins RCC1 protein, human

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Fleischmann M

Institut für Molekularbiologie I, Universität Zürich, Switzerland.

Clark M W

Forrester W

Wickens M

Nishimoto T

Aebi M

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00273932

Published

1991-07-00

Pages

417-23

Language

English

Region

Germany

NLM ID

0125036

Subset

Grants

NIGMS NIH HHS · GM00521 · United States

NIGMS NIH HHS · GM31892 · United States

External Links

PubMed source DOI: 10.1007/BF00273932

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Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation.

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