Rapid identification of specific genes in E. coli by hybridization to membranes containing the ordered set of phage clones.

BioTechniques ·Vol. 10 ·No. 4 ·1991-04-00 ·Pages 474, 476-7

Noda A, Courtright JB, Denor PF, Webb G, Kohara Y, Ishihama A

Abstract

A simplified protocol for the hybridization of labeled oligonucleotides and other radiolabeled DNAs to membranes is described and used for the detection of specific recombinant phage in the ordered miniset collection representing virtually the entire E. coli genome.

Related Genes

gyrA

MeSH Terms

Base Sequence Coliphages/genetics DNA, Recombinant/analysis DNA, Viral/analysis Escherichia coli/genetics Genes, Bacterial Membranes, Artificial Molecular Sequence Data Nucleic Acid Hybridization

Chemicals

DNA, Recombinant DNA, Viral Membranes, Artificial

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Noda A

Biotechnology Research Labs, Takara Shuzo Co., Ltd., Shiga, Japan.

Courtright J B

Denor P F

Webb G

Kohara Y

Ishihama A

Article Info

Journal

BioTechniques

Abbr.

Biotechniques

ISSN

0736-6205

Published

1991-04-00

Pages

474, 476-7

Language

English

Region

England

NLM ID

8306785

Subset

External Links

PubMed source View journal details

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Rapid identification of specific genes in E. coli by hybridization to membranes containing the ordered set of phage clones.

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