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PMID: 18683212 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of glycolysis and oxidative phosphorylation as energy sources for mammalian sperm motility, using the combination of fluorescence imaging, laser tweezers, and real-time automated tracking and trapping.

Journal of cellular physiology ·Vol. 217 ·No. 3 ·2008-12-00 ·Pages 745-51

Nascimento JM, Shi LZ, Tam J, Chandsawangbhuwana C, Durrant B, Botvinick EL, Berns MW

Abstract

The combination of laser tweezers, fluorescent imaging, and real-time automated tracking and trapping (RATTS) can measure sperm swimming speed and swimming force simultaneously with mitochondrial membrane potential (MMP). This approach is used to study the roles of two sources of ATP in sperm motility: oxidative phosphorylation, which occurs in the mitochondria located in the sperm midpiece and glycolysis, which occurs along the length of the sperm tail (flagellum). The relationships between (a) swimming speed and MMP and (b) swimming force and MMP are studied in dog and human sperm. The effects of glucose, oxidative phosphorylation inhibitors and glycolytic inhibitors on human sperm motility are examined. The results indicate that oxidative phosphorylation does contribute some ATP for human sperm motility, but not enough to sustain high motility. The glycolytic pathway is shown to be a primary source of energy for human sperm motility.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Antimycin A/pharmacology Culture Media Dogs Glucose/pharmacology Glycolysis/drug effects Humans Male Membrane Potential, Mitochondrial/drug effects Microscopy, Fluorescence Optical Tweezers Oxidative Phosphorylation/drug effects Rotenone/pharmacology Sperm Motility/drug effects,physiology
Chemicals
Culture Media Rotenone Antimycin A Adenosine Triphosphate Glucose
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nascimento Jaclyn M
Department of Electrical and Computer Engineering, University of California, San Diego, La Jolla, California 92039, USA. [email protected]
Shi Linda Z
Tam James
Chandsawangbhuwana Charlie
Durrant Barbara
Botvinick Elliot L
Berns Michael W
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Article Info
Journal
Journal of cellular physiology
Abbr.
J Cell Physiol
ISSN
1097-4652
DOI
10.1002/jcp.21549
Email
[email protected]
Published
2008-12-00
Pages
745-51
Language
English
Region
United States
NLM ID
0050222
PMCID
PMC3501448
Subset
IM
Grants
NIBIB NIH HHS · P41 EB015890 · United States
NCRR NIH HHS · P41 RR001192 · United States
External Links
PubMed source DOI: 10.1002/jcp.21549 PMC Full Text View journal details
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