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PMID: 1870987 Published · ppublish English Journal Article

PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.

Nucleic acids research ·Vol. 19 ·No. 15 ·1991-08-11 ·Pages 4300

Fung MC, Fung KY

Abstract

暂无摘要

MeSH Terms
Actins/genetics Animals Base Sequence Cell Line DNA, Single-Stranded/genetics DNA-Directed DNA Polymerase/metabolism Interleukin-2/genetics Interleukin-3/genetics Mice Molecular Sequence Data Phenotype Polymerase Chain Reaction RNA, Messenger/genetics Taq Polymerase Temperature
Chemicals
Actins DNA, Single-Stranded Interleukin-2 Interleukin-3 RNA, Messenger Taq Polymerase DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fung M C
Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra.
Fung K Y
References (5)
5 references, click to expand
  1. Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.
    Science. 1988 Aug 5;241(4866):708-12 PMID: 3041594
  2. Preparation of crude cell extract suitable for amplification of RNA by the polymerase chain reaction.
    Nucleic Acids Res. 1989 Mar 11;17(5):2141 PMID: 2928127
  3. The genes for leukemia inhibitory factor and interleukin-6 are expressed in mouse blastocysts prior to the onset of hemopoiesis.
    Mol Cell Biol. 1990 Sep;10(9):4953-6 PMID: 2117704
  4. Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos.
    Science. 1988 Sep 30;241(4874):1823-5 PMID: 3175624
  5. Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.
    Biotechniques. 1989 Nov-Dec;7(10):1096-103 PMID: 2698190
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.15.4300
Published
1991-08-11
Pages
4300
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328586
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.15.4300 PMC Full Text View journal details
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