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PMID: 18719093 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Pathogen corruption and site-directed recombination at a plant disease resistance gene cluster.

Genome research ·Vol. 18 ·No. 12 ·2008-12-00 ·Pages 1918-23

Nagy ED, Bennetzen JL

Abstract

The Pc locus of sorghum (Sorghum bicolor) determines dominant sensitivity to a host-selective toxin produced by the fungal pathogen Periconia circinata. The Pc region was cloned by a map-based approach and found to contain three tandemly repeated genes with the structures of nucleotide binding site-leucine-rich repeat (NBS-LRR) disease resistance genes. Thirteen independent Pc-to-pc mutations were analyzed, and each was found to remove all or part of the central gene of the threesome. Hence, this central gene is Pc. Most Pc-to-pc mutations were associated with unequal recombination. Eight recombination events were localized to different sites in a 560-bp region within the approximately 3.7-kb NBS-LRR genes. Because any unequal recombination located within the flanking NBS-LRR genes would have removed Pc, the clustering of cross-over events within a 560-bp segment indicates that a site-directed recombination process exists that specifically targets unequal events to generate LRR diversity in NBS-LRR loci.

MeSH Terms

Ascomycota/pathogenicity DNA, Plant/genetics,isolation & purification Gene Library Gene Rearrangement Genes, Plant Immunity, Innate Multigene Family Mutagenesis, Site-Directed Plant Diseases/genetics Recombination, Genetic Sequence Analysis, DNA Sorghum/genetics,metabolism

Chemicals

DNA, Plant

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Nagy Ervin D

Department of Genetics, University of Georgia, Athens, Georgia 30602, USA.

Bennetzen Jeffrey L

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Article Info

Journal

Genome research

Abbr.

Genome Res

ISSN

1088-9051

DOI

10.1101/gr.078766.108

Published

2008-12-00

Epub

2008-00-21

Pages

1918-23

Language

English

Region

United States

NLM ID

9518021

PMCID

PMC2593579

Subset

External Links

PubMed source DOI: 10.1101/gr.078766.108 PMC Full Text View journal details

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Pathogen corruption and site-directed recombination at a plant disease resistance gene cluster.

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