Home LiteratureArticle Details
PMID: 1874786 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule.

The Journal of cell biology ·Vol. 114 ·No. 5 ·1991-09-00 ·Pages 1059-68

Albelda SM, Muller WA, Buck CA, Newman PJ

Abstract

PECAM-1 is a 130-120-kD integral membrane glycoprotein found on the surface of platelets, at endothelial intercellular junctions in culture, and on cells of myeloid lineage. Previous studies have shown that it is a member of the immunoglobulin gene superfamily and that antibodies against the bovine form of this protein (endoCAM) can inhibit endothelial cell-cell interactions. These data suggest that PECAM-1 may function as a vascular cell adhesion molecule. The function of this molecule has been further evaluated by transfecting cells with a full-length PECAM-1 cDNA. Transfected COS-7, mouse 3T3 and L cells expressed a 130-120-kD glycoprotein on their cell surface that reacted with anti-PECAM-1 polyclonal and monoclonal antibodies. COS-7 and 3T3 cell transfectants formed cell-cell junctions that were highly enriched in PECAM-1, reminiscent of its distribution at endothelial cell-cell borders. In contrast, this protein remained diffusely distributed within the plasma membrane of PECAM-1 transfected cells that were in contact with mock transfectants. Mouse L cells stably transfected with PECAM-1 demonstrated calcium-dependent aggregation that was inhibited by anti-PECAM antibodies. These results demonstrate that PECAM-1 mediates cell-cell adhesion and support the idea that it may be involved in some of the interactive events taking place during thrombosis, wound healing, and angiogenesis.

MeSH Terms
Amino Acid Sequence Animals Antigens, CD/physiology Antigens, Differentiation, Myelomonocytic/physiology Base Sequence Blood Platelets/cytology Blotting, Northern Calcium/physiology Cell Adhesion Molecules/physiology Cell Aggregation Chlorocebus aethiops Cloning, Molecular Endothelium, Vascular/cytology Fluorescent Antibody Technique Gene Expression Humans In Vitro Techniques Intercellular Junctions/ultrastructure Mice Molecular Sequence Data Platelet Endothelial Cell Adhesion Molecule-1 RNA, Messenger/genetics Transfection
Chemicals
Antigens, CD Antigens, Differentiation, Myelomonocytic Cell Adhesion Molecules Platelet Endothelial Cell Adhesion Molecule-1 RNA, Messenger Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Albelda S M
Wistar Institute, Philadelphia, Pennsylvania 19104.
Muller W A
Buck C A
Newman P J
References (50)
50 references, click to expand
  1. Integrins: a family of cell surface receptors.
    Cell. 1987 Feb 27;48(4):549-54 PMID: 3028640
  2. Integrins and other cell adhesion molecules.
    FASEB J. 1990 Aug;4(11):2868-80 PMID: 2199285
  3. Molecular cloning of CD31, a putative intercellular adhesion molecule closely related to carcinoembryonic antigen.
    J Exp Med. 1990 Jun 1;171(6):2147-52 PMID: 2351935
  4. EndoCAM: a novel endothelial cell-cell adhesion molecule.
    J Cell Biol. 1990 Apr;110(4):1227-37 PMID: 2182647
  5. PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily.
    Science. 1990 Mar 9;247(4947):1219-22 PMID: 1690453
  6. An alternative leukocyte homotypic adhesion mechanism, LFA-1/ICAM-1-independent, triggered through the human VLA-4 integrin.
    J Cell Biol. 1990 Jun;110(6):2157-65 PMID: 1693625
  7. Role of myelin P0 protein as a homophilic adhesion molecule.
    Nature. 1990 Apr 26;344(6269):871-2 PMID: 1691824
  8. VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA-4/fibronectin binding site.
    Cell. 1990 Feb 23;60(4):577-84 PMID: 1689216
  9. The arrangement of the immunoglobulin-like domains of ICAM-1 and the binding sites for LFA-1 and rhinovirus.
    Cell. 1990 Apr 20;61(2):243-54 PMID: 1970514
  10. Membrane proteins involved in phagocyte adherence to endothelium.
    Immunol Rev. 1990 Apr;114:5-28 PMID: 2196221
  11. Leukocyte adhesion to endothelium in inflammation.
    Cell. 1990 Jul 13;62(1):3-6 PMID: 2194672
  12. Warner-Lambert/Parke-Davis Award lecture. Cellular and molecular mechanisms that direct leukocyte traffic.
    Am J Pathol. 1990 Jan;136(1):3-11 PMID: 2404417
  13. Drosophila fasciclin I is a novel homophilic adhesion molecule that along with fasciclin III can mediate cell sorting.
    J Cell Biol. 1990 May;110(5):1825-32 PMID: 2335571
  14. The neural cell adhesion molecule N-CAM enhances L1-dependent cell-cell interactions.
    J Cell Biol. 1990 Jan;110(1):193-208 PMID: 2295682
  15. Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion.
    EMBO J. 1984 Jan;3(1):1-10 PMID: 6368220
  16. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  17. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  18. Neuronal cell-cell adhesion depends on interactions of N-CAM with heparin-like molecules.
    Nature. 1986 Apr 3-9;320(6061):445-7 PMID: 3083264
  19. Morphoregulatory molecules.
    Biochemistry. 1988 May 17;27(10):3533-43 PMID: 3044445
  20. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.
    Development. 1988 Apr;102(4):639-55 PMID: 3048970
  21. The immunoglobulin superfamily--domains for cell surface recognition.
    Annu Rev Immunol. 1988;6:381-405 PMID: 3289571
  22. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation.
    Cell. 1989 Mar 24;56(6):1033-44 PMID: 2466574
  23. Molecular modeling of protein-glycosaminoglycan interactions.
    Arteriosclerosis. 1989 Jan-Feb;9(1):21-32 PMID: 2463827
  24. Structural requirements for neural cell adhesion molecule-heparin interaction.
    Cell Regul. 1990 Jul;1(8):567-76 PMID: 2078567
  25. A mouse carcinoembryonic antigen gene family member is a calcium-dependent cell adhesion molecule.
    J Biol Chem. 1991 Jan 5;266(1):309-15 PMID: 1985902
  26. Molecular characterization and functional analysis of the leukocyte surface protein CD31.
    J Immunol. 1990 Dec 1;145(11):3889-97 PMID: 1700999
  27. Carbohydrate ligands of the LEC cell adhesion molecules.
    Cell. 1990 Nov 30;63(5):861-3 PMID: 1701693
  28. Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism.
    J Exp Med. 1991 Feb 1;173(2):515-8 PMID: 1671083
  29. Cadherin cell adhesion receptors as a morphogenetic regulator.
    Science. 1991 Mar 22;251(5000):1451-5 PMID: 2006419
  30. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes.
    Cell. 1989 Oct 20;59(2):305-12 PMID: 2478294
  31. Neuron-glia cell adhesion molecule interacts with neurons and astroglia via different binding mechanisms.
    J Cell Biol. 1988 Feb;106(2):487-503 PMID: 2448316
  32. Myelin-associated glycoprotein, a member of the L2/HNK-1 family of neural cell adhesion molecules, is involved in neuron-oligodendrocyte and oligodendrocyte-oligodendrocyte interaction.
    J Cell Biol. 1987 Oct;105(4):1893-9 PMID: 2444603
  33. Expressed recombinant cadherins mediate cell sorting in model systems.
    Cell. 1988 Sep 23;54(7):993-1001 PMID: 3416359
  34. Diversity of the immunoglobulin gene superfamily.
    Adv Immunol. 1989;44:1-63 PMID: 2646860
  35. New perspectives in cell adhesion: RGD and integrins.
    Science. 1987 Oct 23;238(4826):491-7 PMID: 2821619
  36. Cell surface receptors for extracellular matrix molecules.
    Annu Rev Cell Biol. 1987;3:179-205 PMID: 2825736
  37. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins.
    Science. 1989 Mar 3;243(4895):1160-5 PMID: 2466335
  38. A heparin-binding domain from N-CAM is involved in neural cell-substratum adhesion.
    J Cell Biol. 1986 Feb;102(2):403-12 PMID: 2418031
  39. A novel leukocyte differentiation antigen: two monoclonal antibodies TM2 and TM3 define a 120-kd molecule present on neutrophils, monocytes, platelets, and activated lymphoblasts.
    Blood. 1985 Oct;66(4):873-81 PMID: 2412619
  40. Characterization of a novel myeloid antigen regulated during differentiation of monocytic cells.
    Eur J Immunol. 1989 Aug;19(8):1373-8 PMID: 2506060
  41. The role of the LFA-1/ICAM-1 interaction in human leukocyte homing and adhesion.
    Immunol Rev. 1989 Apr;108:135-61 PMID: 2670740
  42. Expression of normal and mutant avian integrin subunits in rodent cells.
    J Cell Biol. 1989 Aug;109(2):853-61 PMID: 2788168
  43. Identification and characterization of cell-substratum adhesion receptors on cultured human endothelial cells.
    J Clin Invest. 1989 Jun;83(6):1992-2002 PMID: 2786007
  44. Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule.
    Cell. 1989 Apr 21;57(2):327-34 PMID: 2702691
  45. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  46. Biochemistry and expression of myelomonocytic antigens.
    J Immunol. 1986 Dec 15;137(12):3909-14 PMID: 3465828
  47. Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing.
    Science. 1987 May 15;236(4803):799-806 PMID: 3576199
  48. Direct demonstration of the lectin activity of gp90MEL, a lymphocyte homing receptor.
    J Cell Biol. 1990 Sep;111(3):1225-32 PMID: 2202735
  49. Expression of CD44 confers a new adhesive phenotype on transfected cells.
    Cell. 1990 Jan 12;60(1):45-52 PMID: 2403843
  50. Vascular endothelial cells synthesize a plasma membrane protein indistinguishable from the platelet membrane glycoprotein IIa.
    J Biol Chem. 1985 Sep 15;260(20):11300-6 PMID: 3161894
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.114.5.1059
Published
1991-09-00
Pages
1059-68
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289123
Subset
IM
Grants
NCI NIH HHS · CA10815 · United States
NHLBI NIH HHS · HL-01587 · United States
NHLBI NIH HHS · HL-39023 · United States
Databases
GENBANK
M28526, M60691, M60692, M60693, M60694, M60695, M60696, M60697, S50260, X56699, X57971
External Links
PubMed source DOI: 10.1083/jcb.114.5.1059 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2