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PMID: 18757937 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A SUMO-like domain protein, Esc2, is required for genome integrity and sister chromatid cohesion in Saccharomyces cerevisiae.

Genetics ·Vol. 180 ·No. 1 ·2008-09-00 ·Pages 41-50

Ohya T, Arai H, Kubota Y, Shinagawa H, Hishida T

Abstract

The ESC2 gene encodes a protein with two tandem C-terminal SUMO-like domains and is conserved from yeasts to humans. Previous studies have implicated Esc2 in gene silencing. Here, we explore the functional significance of SUMO-like domains and describe a novel role for Esc2 in promoting genome integrity during DNA replication. This study shows that esc2Delta cells are modestly sensitive to hydroxyurea (HU) and defective in sister chromatid cohesion and have a reduced life span, and these effects are enhanced by deletion of the RRM3 gene that is a Pif1-like DNA helicase. esc2Delta rrm3Delta cells also have a severe growth defect and accumulate DNA damage in late S/G2. In contrast, esc2Delta does not enhance the HU sensitivity or sister chromatid cohesion defect in mrc1Delta cells, but rather partially suppresses both phenotypes. We also show that deletion of both Esc2 SUMO-like domains destabilizes Esc2 protein and functionally inactivates Esc2, but this phenotype is suppressed by an Esc2 variant with an authentic SUMO domain. These results suggest that Esc2 is functionally equivalent to a stable SUMO fusion protein and plays important roles in facilitating DNA replication fork progression and sister chromatid cohesion that would otherwise impede the replication fork in rrm3Delta cells.

MeSH Terms
Cell Cycle Cell Cycle Proteins Chromatids/genetics Chromosome Segregation DNA Damage DNA Replication Gene Deletion Gene Silencing Genome, Fungal Hydroxyurea/pharmacology Models, Genetic Nuclear Proteins/genetics,physiology Plasmids/metabolism Recombinant Fusion Proteins/chemistry Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics,physiology Two-Hybrid System Techniques
Chemicals
Cell Cycle Proteins Esc2 protein, S cerevisiae Nuclear Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Hydroxyurea
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ohya Tomoko
Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Arai Hirokazu
Kubota Yoshino
Shinagawa Hideo
Hishida Takashi
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
Published
2008-09-00
Epub
2008-00-30
Pages
41-50
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2535692
Subset
IM
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