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PMID: 1875946 Published · ppublish English Journal Article

Characterization of the major regulatory element upstream of the human alpha-globin gene cluster.

Molecular and cellular biology ·Vol. 11 ·No. 9 ·1991-09-00 ·Pages 4679-89

Jarman AP, Wood WG, Sharpe JA, Gourdon G, Ayyub H, Higgs DR

Abstract

The major positive regulatory activity of the human alpha-globin gene complex has been localized to an element associated with a strong erythroid-specific DNase I hypersensitive site (HS -40) located 40 kb upstream of the zeta 2-globin mRNA cap site. Footprint and gel shift analyses of the element have demonstrated the presence of four binding sites for the nuclear factor GATA-1 and two sites corresponding to the AP-1 consensus binding sequence. This region resembles one of the major elements of the beta-globin locus control region in its constitution and characteristics; this together with evidence from expression studies suggests that HS -40 is a primary element controlling alpha-globin gene expression.

Related Genes
neo<up>r</up>
MeSH Terms
Base Sequence DNA Deoxyribonuclease I/metabolism Drug Resistance/genetics Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Globins/genetics Humans Molecular Sequence Data Multigene Family Neomycin/pharmacology Regulatory Sequences, Nucleic Acid Restriction Mapping Transformation, Genetic
Chemicals
Globins DNA Deoxyribonuclease I Neomycin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jarman A P
MRC Molecular Haematology Unit, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Wood W G
Sharpe J A
Gourdon G
Ayyub H
Higgs D R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.9.4679-4689.1991
Published
1991-09-00
Pages
4679-89
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361359
Subset
IM
Databases
GENBANK
M64827, S43604, S43609, S43613, S43614, S43616, S43620, S49742, S49899, X57834
External Links
PubMed source DOI: 10.1128/mcb.11.9.4679-4689.1991 PMC Full Text View journal details
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