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PMID: 18772282 Published · ppublish English Journal Article Review

Progress in metabolic engineering of Saccharomyces cerevisiae.

Microbiology and molecular biology reviews : MMBR ·Vol. 72 ·No. 3 ·2008-09-00 ·Pages 379-412

Nevoigt E

Abstract

The traditional use of the yeast Saccharomyces cerevisiae in alcoholic fermentation has, over time, resulted in substantial accumulated knowledge concerning genetics, physiology, and biochemistry as well as genetic engineering and fermentation technologies. S. cerevisiae has become a platform organism for developing metabolic engineering strategies, methods, and tools. The current review discusses the relevance of several engineering strategies, such as rational and inverse metabolic engineering, evolutionary engineering, and global transcription machinery engineering, in yeast strain improvement. It also summarizes existing tools for fine-tuning and regulating enzyme activities and thus metabolic pathways. Recent examples of yeast metabolic engineering for food, beverage, and industrial biotechnology (bioethanol and bulk and fine chemicals) follow. S. cerevisiae currently enjoys increasing popularity as a production organism in industrial ("white") biotechnology due to its inherent tolerance of low pH values and high ethanol and inhibitor concentrations and its ability to grow anaerobically. Attention is paid to utilizing lignocellulosic biomass as a potential substrate.

MeSH Terms
Biotechnology Food Industry Genetic Engineering/methods Industrial Microbiology Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Saccharomyces cerevisiae Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Nevoigt Elke
Department of Microbiology and Genetics, Berlin University of Technology, Seestr. 13, 13353 Berlin, Germany. [email protected]
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Article Info
Journal
Microbiology and molecular biology reviews : MMBR
Abbr.
Microbiol Mol Biol Rev
ISSN
1098-5557
DOI
10.1128/MMBR.00025-07
Email
[email protected]
Published
2008-09-00
Pages
379-412
Language
English
Region
United States
NLM ID
9706653
PMCID
PMC2546860
Subset
IM
External Links
PubMed source DOI: 10.1128/MMBR.00025-07 PMC Full Text View journal details
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