The location of the feedback-specific region with the pyrimidine-3 locus of Neurospora crassa.

Molecular & general genetics : MGG ·Vol. 146 ·No. 3 ·1976-08-02 ·Pages 247-51

Makoff AJ, Radford A

Abstract

The purimidine-3 locus of Neurospora crassa specifies two enzyme activities, pyrimidine-specific carbamyl phosphate synthetase (CPSpyr) and aspartate transcarbamylase (ATC). ATC is translationally distal. CPSpyr, but not ATC, is subject to feedback inhibition by uridine triphosphate (UTP). To investigate the location of the feedback-specific region within the locus, inhibition of a number of pyr-3 alleles by UTP was investigated. All CPS+ ATC- polar alleles, revertants of CPS- ATC- polar alleles, and 5-fluorouracil-resistant mutants had normal UTP response. The location of the feedback-specific region is in or close to the CPS-specific region.

MeSH Terms

Aspartate Carbamoyltransferase Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) Chromosome Mapping Feedback Genes Genetic Techniques Neurospora/enzymology Neurospora crassa/enzymology Peptide Chain Termination, Translational Phosphotransferases Pyrimidines/metabolism Uracil Nucleotides/pharmacology

Chemicals

Pyrimidines Uracil Nucleotides Aspartate Carbamoyltransferase Phosphotransferases Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Makoff A J

Radford A

References (17)

17 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00701247

Published

1976-08-02

Pages

247-51

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00701247

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The location of the feedback-specific region with the pyrimidine-3 locus of Neurospora crassa.

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