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PMID: 1879944 Published · ppublish English Journal Article

Pathogenicity of Candida albicans auxotrophic mutants in experimental infections.

Infection and immunity ·Vol. 59 ·No. 9 ·1991-09-00 ·Pages 3297-300

Kirsch DR, Whitney RR

Abstract

Auxotrophic and prototrophic control strain pairs of Candida albicans constructed by molecular biology methodologies were evaluated for pathogenicity in a systemic mouse model. Mutants that were auxotrophic for adenine, uracil, and heme each showed a lowered level of pathogenicity relative to control strains. It can be concluded from these experiments that decreased pathogenicity in each case is due to the auxotrophic mutation, because mutant and control strains were constructed so as to differ at a single locus. These observations suggest that new therapeutic agents for Candida infections might be designed based upon the inhibition of biosynthetic pathways that, in some cases, might be absent from the host.

MeSH Terms
Animals Candida albicans/genetics,pathogenicity Candidiasis/microbiology Disease Models, Animal Female Genotype Mice Mutation Virulence
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kirsch D R
Department of Microbial Biochemistry and Genetics, Squibb Institute for Medical Research, Princeton, New Jersey 08540.
Whitney R R
References (25)
25 references, click to expand
  1. PROMOTION OF RETARDATION OF THE GROWTH OF ADENINE AUXOTROPHS OF CANDIDA ALBICANS BY PURINES, PYRIMIDINES AND NUCLEOSIDES.
    Antonie Van Leeuwenhoek. 1964;30:289-302 PMID: 14218441
  2. Studies on the pink, adenine-deficient strains of Candida albicans. I. Cultural and morphological characteristics.
    Sabouraudia. 1970 May;8(1):48-59 PMID: 5427154
  3. Isolation of hem3 mutants from Candida albicans by sequential gene disruption.
    Mol Gen Genet. 1989 May;217(1):47-52 PMID: 2671651
  4. Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene.
    Mol Cell Biol. 1986 Jan;6(1):142-9 PMID: 3023819
  5. Development of autonomously replicating plasmids for Candida albicans.
    Mol Cell Biol. 1987 Jan;7(1):209-17 PMID: 3031460
  6. Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants.
    Mol Cell Biol. 1987 Jan;7(1):199-208 PMID: 3031459
  7. One-step gene disruption by cotransformation to isolate double auxotrophs in Candida albicans.
    Mol Gen Genet. 1988 Sep;214(1):24-31 PMID: 2976118
  8. The molecular genetics of Candida albicans.
    Microbiol Sci. 1988 Feb;5(2):58-63 PMID: 3079219
  9. Isolation of the Candida albicans gene for orotidine-5'-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations.
    Mol Gen Genet. 1984;198(2):179-82 PMID: 6394964
  10. Candida albicans proteinase as a virulence factor in the pathogenesis of Candida infections.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Dec;260(4):539-42 PMID: 3914794
  11. Pathogenicity of morphological and auxotrophic mutants of Candida albicans in experimental infections.
    Infect Immun. 1985 Nov;50(2):541-4 PMID: 3902649
  12. Yeast adhesion in the pathogenesis of endocarditis due to Candida albicans: studies with adherence-negative mutants.
    J Infect Dis. 1985 Oct;152(4):710-5 PMID: 3900233
  13. Correlative relationship between adherence of Candida albicans to human vaginal epithelial cells in vitro and candidal vaginitis.
    Sabouraudia. 1984;22(3):191-200 PMID: 6379915
  14. Heterozygosity and segregation in Candida albicans.
    Mol Gen Genet. 1980;180(1):107-13 PMID: 7003306
  15. Critical role of germ tube formation in the pathogenesis of candidal vaginitis.
    Infect Immun. 1984 Jun;44(3):576-80 PMID: 6327527
  16. Comparative pathogenicity of auxotrophic mutants of Candida albicans.
    Can J Microbiol. 1984 Jan;30(1):31-5 PMID: 6370397
  17. Genetic analysis of red, adenine-requiring mutants of Candida albicans.
    J Bacteriol. 1983 Dec;156(3):1066-77 PMID: 6358187
  18. Protoplast fusion hybrids of Candida albicans sterol mutants differing in nystatin resistance.
    J Gen Microbiol. 1982 Jan;128(1):123-8 PMID: 7045281
  19. Toxins produced by Candida albicans.
    Contrib Microbiol Immunol. 1977;4:77-85 PMID: 342194
  20. Phospholipase activity in Candida albicans.
    Sabouraudia. 1977 Jul;15(2):179-85 PMID: 333624
  21. Porphyrine mutants of Saccharomyces cerevisiae: correlated lesions in sterol and fatty acid biosynthesis.
    Biochem Biophys Res Commun. 1974 Jan 23;56(2):324-30 PMID: 4596057
  22. Biological and chemical characterization of toxic substances from Candida albicans.
    Infect Immun. 1972 Oct;6(4):616-27 PMID: 4564290
  23. The cytochemical localization of phospholipase a and lysophospholipase in Candida albicans.
    Sabouraudia. 1975 Mar;13 Pt 1:110-5 PMID: 1091995
  24. An examination of the production of hydrolytic enzymes and toxins by pathogenic strains of Candida albicans.
    J Gen Microbiol. 1971 Aug;67(3):255-63 PMID: 5137675
  25. Candida proteinases and candidosis.
    Crit Rev Biotechnol. 1988;8(2):121-9 PMID: 3067860
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.9.3297-3300.1991
Published
1991-09-00
Pages
3297-300
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258168
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.59.9.3297-3300.1991 PMC Full Text View journal details
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