Nucleotide sequence of the restriction fragments hind L and hind M of SV40 DNA.

Nucleic acids research ·Vol. 3 ·No. 12 ·1976-12-00 ·Pages 3409-21

Ysebaert M, Thys F, Van de Voorde A, Fiers W

Abstract

The nucleotide sequence of the two minor SV40 DNA restriction fragments Hind L and Hind M is here reported. For this purpose we used 5'-32p-labeled DNA fragments either partially digested with snake venom diesterase for analysis by the wandering spot method or partially degraded with the base specific reagents dimethylsulphate or hydrazine for direct sequence analysis on gel. In the former procedure the strands were separated before degradation, while in the latter the strands were separated after modification but before the degradation. Due to the presence of several termination codons, the region Hind L - Hind M cannot be translated in a polypeptide. Also, no initiation codons for protein synthesis are present in this region.

MeSH Terms

Base Sequence Codon DNA Restriction Enzymes DNA, Viral/analysis Haemophilus/enzymology Simian virus 40/analysis

Chemicals

Codon DNA, Viral DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Ysebaert M

Thys F

Van de Voorde A

Fiers W

References (10)

10 references, click to expand

Location of the small restriction fragments, Hind-L, Hind-M and Hpa-E, on the simian virus 40 genome.
Virology. 1975 Nov;68(1):260-5 PMID: 171849
Cleavage map of the simian-virus-40 genome by the restriction endonuclease III of Haemopholus aegyptius.
Eur J Biochem. 1976 Jan 2;61(1):101-17 PMID: 173548
Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.
J Mol Biol. 1973 Aug 5;78(2):363-76 PMID: 4355833
Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.
Biochem Biophys Res Commun. 1974 Jul 10;59(1):108-16 PMID: 4366956
Viable deletion mutants of simian virus 40: selective isolation by means of a restriction endonuclease from Hemophilus parainfluenzae.
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4879-83 PMID: 4373732
Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
A restriction enzyme from Hemophilus influenzae. II.
J Mol Biol. 1970 Jul 28;51(2):393-409 PMID: 5312501
Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 PMID: 5323016
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
Biochem J. 1964 Jan;90(1):147-9 PMID: 5832284
Analysis of 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure.
Anal Biochem. 1976 May 7;72:433-46 PMID: 942063

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.12.3409

Published

1976-12-00

Pages

3409-21

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343185

Subset

Databases

GENBANK

J02400, J02402, J02403, J02406, J02407, J02408, J02409, J02410, V01380

External Links

PubMed source DOI: 10.1093/nar/3.12.3409 PMC Full Text View journal details

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Nucleotide sequence of the restriction fragments hind L and hind M of SV40 DNA.

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