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PMID: 18818395 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The CD34-like protein PODXL and alpha6-integrin (CD49f) identify early progenitor MSCs with increased clonogenicity and migration to infarcted heart in mice.

Blood ·Vol. 113 ·No. 4 ·2009-01-22 ·Pages 816-26

Lee RH, Seo MJ, Pulin AA, Gregory CA, Ylostalo J, Prockop DJ

Abstract

We screened for surface proteins expressed only by the early progenitor cells present in low-passage, low-density cultures of the adult stem/progenitor cells from bone marrow referred to as mesenchymal stem cells or multipotent stromal cells (MSCs). Six proteins were identified that were selectively expressed in the early progenitors: podocalyxin-like protein (PODXL), alpha6-integrin (CD49f), alpha4-integrin (CD49d), c-Met, CXCR4, and CX3CR1. All were previously shown to be involved in cell trafficking or tumor progression. Antibodies to CD49f and PODXL, a sialomucin in the CD34 family, were the most robust for FACScan assays. PODXL(hi)/CD49f(hi) MSCs were more clonogenic and differentiated more efficiently than PODXL(lo)/CD49f(lo) cells. Inhibition of expression of PODXL with RNA interference caused aggregation of the cells. Furthermore, PODXL(hi)/CD49f(hi) MSCs were less prone to produce lethal pulmonary emboli, and larger numbers were recovered in heart and kidney after intravenous infusion into mice with myocardial infarcts.

MeSH Terms
Animals Antigens, CD34/metabolism Cell Movement Cells, Cultured Epitopes/immunology Gene Expression Regulation Humans Integrin alpha6/genetics,metabolism Kidney/cytology,metabolism Mesenchymal Stem Cells/cytology,immunology,metabolism Mice Myocardial Infarction/immunology,metabolism Oligonucleotide Array Sequence Analysis RNA Interference Sialoglycoproteins/genetics,metabolism Time Factors
Chemicals
Antigens, CD34 Epitopes Integrin alpha6 Sialoglycoproteins podocalyxin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lee Ryang Hwa
Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA, USA.
Seo Min Jeong
Pulin Andrey A
Gregory Carl A
Ylostalo Joni
Prockop Darwin J
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Article Info
Journal
Blood
Abbr.
Blood
ISSN
1528-0020
DOI
10.1182/blood-2007-12-128702
Published
2009-01-22
Epub
2008-00-25
Pages
816-26
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC2630267
Subset
IM
Grants
NHLBI NIH HHS · HL073252 · United States
NHLBI NIH HHS · HL 073755 · United States
NHLBI NIH HHS · P01 HL075161 · United States
NHLBI NIH HHS · R01 HL073755 · United States
NHLBI NIH HHS · P01 HL 075161 · United States
NHLBI NIH HHS · R01 HL073252 · United States
External Links
PubMed source DOI: 10.1182/blood-2007-12-128702 PMC Full Text View journal details
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