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PMID: 18840611 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

O-linked beta-N-acetylglucosaminyltransferase substrate specificity is regulated by myosin phosphatase targeting and other interacting proteins.

The Journal of biological chemistry ·Vol. 283 ·No. 49 ·2008-12-05 ·Pages 33935-41

Cheung WD, Sakabe K, Housley MP, Dias WB, Hart GW

Abstract

O-GlcNAc-transferase (OGT) substrate specificity is regulated by transiently interacting proteins. To further examine the regulation of OGT, we have identified 27 putative OGT-interacting proteins through a yeast two-hybrid screen. Two of these proteins, Trak1 (OIP106) and O-GlcNAcase, have been shown previously to interact with and regulate OGT. We demonstrate here that MYPT1 and CARM1 also interact with and target OGT. MYPT1 and CARM1 are substrates of OGT in vitro and in vivo. MYPT1 and CARM1 also function to alter OGT substrate specificity in vitro. Furthermore depletion of MYPT1 in Neuro-2a neuroblastoma cells alters GlcNAcylation of several proteins under basal conditions, suggesting that MYPT1 regulates OGT substrate specificity in vivo.

MeSH Terms
Adaptor Proteins, Vesicular Transport Animals Carrier Proteins/physiology Cell Line, Tumor Gene Expression Regulation, Enzymologic Humans Mice Models, Biological Myosin-Light-Chain Kinase/physiology Myosin-Light-Chain Phosphatase/chemistry N-Acetylglucosaminyltransferases/chemistry Protein Binding Protein Structure, Tertiary RNA Polymerase II/chemistry Substrate Specificity Two-Hybrid System Techniques
Chemicals
Adaptor Proteins, Vesicular Transport Carrier Proteins Trak1 protein, mouse N-Acetylglucosaminyltransferases Myosin-Light-Chain Kinase RNA Polymerase II Myosin-Light-Chain Phosphatase Ppp1r12a protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cheung Win D
Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2185, USA.
Sakabe Kaoru
Housley Michael P
Dias Wagner B
Hart Gerald W
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
DOI
10.1074/jbc.M806199200
Published
2008-12-05
Epub
2008-00-07
Pages
33935-41
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2590692
Subset
IM
Grants
NCI NIH HHS · R01 CA42486 · United States
NICHD NIH HHS · R01 HD13563 · United States
External Links
PubMed source DOI: 10.1074/jbc.M806199200 PMC Full Text View journal details
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