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PMID: 1889880 Published · ppublish English Journal Article

Investigations of the frequency of DNA strand breakage and cross-linking and of sister chromatid exchange in the lymphocytes of electric welders exposed to chromium- and nickel-containing fumes.

International archives of occupational and environmental health ·Vol. 63 ·No. 2 ·1991-00-00 ·Pages 115-20

Popp W, Vahrenholz C, Schmieding W, Krewet E, Norpoth K

Abstract

A total of 39 electric welders exposed to chromium and nickel were compared with 18 controls standardized for age, smoking habits and sex with respect to the frequency of sister chromatid exchange (SCE) and of DNA strand breakage and cross-linking (measured by the method of alkaline filter elution) in their blood lymphocytes. A significant correlation was found between the frequency of SCE and of individual DNA strand breakage and the concentration of chromium in the urine. Less DNA from the welders than from the control group was eluted through the two filter types used (polycarbonate and polyvinylidene fluoride filters). This must be interpreted as resulting from the presence of DNA-protein cross-links, which has the secondary effect of leading to a relative reduction in the measurable frequency of strand breakage amongst the welders. The present results are in good agreement with in vitro and in vivo investigations that confirm the importance of DNA-protein cross-links for the carcinogenic effect of chromium.

MeSH Terms
Air Pollutants, Occupational/toxicity Animals Cell Line Chromium/pharmacokinetics,toxicity Cricetinae DNA Damage Environmental Monitoring Humans Male Nickel/pharmacokinetics,toxicity Occupational Exposure Risk Factors Sister Chromatid Exchange/drug effects,genetics Smoking/adverse effects,genetics Welding
Chemicals
Air Pollutants, Occupational Chromium Nickel
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Popp W
Institute for Hygiene and Industrial Medicine, University of Essen (GHS), Federal Republic of Germany.
Vahrenholz C
Schmieding W
Krewet E
Norpoth K
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Article Info
Journal
International archives of occupational and environmental health
Abbr.
Int Arch Occup Environ Health
ISSN
0340-0131
DOI
10.1007/BF00379074
Published
1991-00-00
Pages
115-20
Language
English
Region
Germany
NLM ID
7512134
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00379074 View journal details
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