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PMID: 1893102 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

cDNA cloning, characterization and expression of an endosperm-specific barley peroxidase.

Plant molecular biology ·Vol. 16 ·No. 2 ·1991-02-00 ·Pages 317-27

Rasmussen SK, Welinder KG, Hejgaard J

Abstract

A barley peroxidase (BP 1) of pI ca. 8.5 and Mr 37,000 has been purified from mature barley grains. Using antibodies towards peroxidase BP 1, a cDNA clone (pcR7) was isolated from a cDNA expression library. The nucleotide sequence of pcR7 gave a derived amino acid sequence identical to the 158 C-terminal amino acid residues of mature BP 1. The clone pcR7 encodes an additional C-terminal sequence of 22 residues, which apparently are removed during processing. BP 1 is less than 50% identical to other sequenced plant peroxidases. Analyses of RNA and protein from aleurone, endosperm and embryo tissue showed maximal expression 15 days after flowering, and high levels were found only in the endosperm. BP 1 was not expressed in the leaves.

MeSH Terms

Amino Acid Sequence Cloning, Molecular DNA DNA Probes Hordeum/enzymology,genetics Molecular Sequence Data Peroxidases/biosynthesis,genetics Plant Proteins/biosynthesis,genetics,isolation & purification Sequence Alignment

Chemicals

DNA Probes Plant Proteins DNA Peroxidases barley peroxidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rasmussen S K

Plant Biology Section, Risø National Laboratory, Roskilde, Denmark.

Welinder K G

Hejgaard J

References (25)

25 references, click to expand

Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
DNA. 1985 Apr;4(2):165-70 PMID: 3996185
Isolation and properties of basic isoenzymes of horseradish peroxidase.
J Biochem. 1981 Aug;90(2):489-96 PMID: 7298597
Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase.
Mol Gen Genet. 1989 Jun;217(2-3):223-32 PMID: 2528055
Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression.
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7542-6 PMID: 16593885
Plant peroxidases. Their primary, secondary and tertiary structures, and relation to cytochrome c peroxidase.
Eur J Biochem. 1985 Sep 16;151(3):497-504 PMID: 2992968
A simple and very efficient method for generating cDNA libraries.
Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
Efficient isolation of genes by using antibody probes.
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits.
Plant Mol Biol. 1988 Jan;11(1):15-26 PMID: 24272154
Covalent structure of turnip peroxidase 7. Cyanogen bromide fragments, complete structure and comparison to horseradish peroxidase C.
Eur J Biochem. 1980 Jul;108(2):481-9 PMID: 7408864
Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose.
Eur J Biochem. 1972 Dec 4;31(2):246-54 PMID: 4647179
Amino acid sequence studies of horseradish peroxidase. Amino and carboxyl termini, cyanogen bromide and tryptic fragments, the complete sequence, and some structural characteristics of horseradish peroxidase C.
Eur J Biochem. 1979 Jun 1;96(3):483-502 PMID: 38113
Synthesis of salt-soluble proteins in barley. Pulse-labeling study of grain filling in liquid-cultured detached spikes.
Planta. 1984 May;161(2):172-7 PMID: 24253606
Isolation and sequencing of cDNA clones encoding ethylene-induced putative peroxidases from cucumber cotyledons.
Plant Mol Biol. 1990 May;14(5):715-25 PMID: 2102850
cDNA cloning, in vitro transcription and partial sequence analysis of mRNAs from winter wheat (Triticum aestivum L.) with induced resistance to Erysiphe graminis f. sp. tritici.
Plant Mol Biol. 1989 Jun;12(6):643-54 PMID: 24271197
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.
J Biochem Biophys Methods. 1984 Dec;10(3-4):203-9 PMID: 6530509
The characterization of cDNA clones coding for wheat storage proteins.
Nucleic Acids Res. 1983 May 25;11(10):2961-77 PMID: 6190127
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Cleavage of the haem-protein link by acid methylethylketone.
Biochim Biophys Acta. 1959 Oct;35:543 PMID: 13837237
Barley leaf peroxidase: purification and characterization.
J Biochem. 1986 Feb;99(2):485-94 PMID: 3009425
Structure of the horseradish peroxidase isozyme C genes.
Eur J Biochem. 1988 May 2;173(3):681-7 PMID: 3371352
The chromosomal locations of leaf peroxidase genes in hexaploid wheat, rye and barley.
Theor Appl Genet. 1984 Dec;69(2):205-10 PMID: 24253713
Amino acid analysis: determination of cysteine plus half-cystine in proteins after hydrochloric acid hydrolysis with a disulfide compound as additive.
Anal Biochem. 1989 Mar;177(2):318-22 PMID: 2729552

Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00020562

Published

1991-02-00

Pages

317-27

Language

English

Region

Netherlands

NLM ID

9106343

Subset

Databases

GENBANK

M73234, S55707, S77862, S77864, S77866, S77870, S77874, S77877, X53384, X57126

External Links

PubMed source DOI: 10.1007/BF00020562 View journal details

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cDNA cloning, characterization and expression of an endosperm-specific barley peroxidase.

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