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PMID: 18936247 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

SPDL-1 functions as a kinetochore receptor for MDF-1 in Caenorhabditis elegans.

The Journal of cell biology ·Vol. 183 ·No. 2 ·2008-10-20 ·Pages 187-94

Yamamoto TG, Watanabe S, Essex A, Kitagawa R

Abstract

The spindle assembly checkpoint (SAC) ensures faithful chromosome segregation by delaying anaphase onset until all sister kinetochores are attached to bipolar spindles. An RNA interference screen for synthetic genetic interactors with a conserved SAC gene, san-1/MAD3, identified spdl-1, a Caenorhabditis elegans homologue of Spindly. SPDL-1 protein localizes to the kinetochore from prometaphase to metaphase, and this depends on KNL-1, a highly conserved kinetochore protein, and CZW-1/ZW10, a component of the ROD-ZW10-ZWILCH complex. In two-cell-stage embryos harboring abnormal monopolar spindles, SPDL-1 is required to induce the SAC-dependent mitotic delay and localizes the SAC protein MDF-1/MAD1 to the kinetochore facing away from the spindle pole. In addition, SPDL-1 coimmunoprecipitates with MDF-1/MAD1 in vivo. These results suggest that SPDL-1 functions in a kinetochore receptor of MDF-1/MAD1 to induce SAC function.

MeSH Terms
Animals Caenorhabditis elegans/cytology,embryology,genetics,metabolism Caenorhabditis elegans Proteins/metabolism Cell Cycle Proteins/metabolism Cell Polarity Centrosome/metabolism Chromosome Segregation Embryo Loss Embryo, Nonmammalian/cytology Genes, Helminth Kinetochores/metabolism Meiosis Protein Binding Protein Transport RNA Interference Spindle Apparatus/metabolism Suppression, Genetic
Chemicals
Caenorhabditis elegans Proteins Cell Cycle Proteins MDF-1 protein, C elegans SPDL-1 protein, C elegans
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamamoto Takaharu G
Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Watanabe Sonoko
Essex Anthony
Kitagawa Risa
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
1540-8140
DOI
10.1083/jcb.200805185
Published
2008-10-20
Pages
187-94
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2568016
Subset
IM
Grants
NCI NIH HHS · P30 CA021765 · United States
NCI NIH HHS · CA021765 · United States
External Links
PubMed source DOI: 10.1083/jcb.200805185 PMC Full Text View journal details
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