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PMID: 18980248 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Adipose triglyceride lipase regulates basal lipolysis and lipid droplet size in adipocytes.

Journal of cellular biochemistry ·Vol. 105 ·No. 6 ·2008-12-15 ·Pages 1430-6

Miyoshi H, Perfield JW, Obin MS, Greenberg AS

Abstract

In adipocytes, lipid droplet (LD) size reflects a balance of triglyceride synthesis (lipogenesis) and hydrolysis (lipolysis). Perilipin A (Peri A) is the most abundant phosphoprotein on the surface of adipocyte LDs and has a crucial role in lipid storage and lipolysis. Adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) are the major rate-determining enzymes for lipolysis in adipocytes. Each of these proteins (Peri A, ATGL, and HSL) has been demonstrated to regulate lipid storage and release in the adipocyte. However, in the absence of protein kinase A (PKA) stimulation (basal state), the lipases (ATGL and HSL) are located mainly in the cytoplasm, and their contribution to basal rates of lipolysis and influence on LD size are poorly understood. In this study, we utilize an adenoviral system to knockdown or overexpress ATGL and HSL in an engineered model system of adipocytes in the presence or absence of Peri A. We are able to demonstrate in our experimental model system that in the basal state, LD size, triglyceride storage, and fatty acid release are mainly influenced by the expression of ATGL. These results demonstrate for the first time the relative contributions of ATGL, HSL, and Peri A on determination of LD size in the absence of PKA stimulation.

MeSH Terms
Adenoviridae/genetics,metabolism Adipocytes/enzymology Animals Carboxylic Ester Hydrolases/metabolism Carrier Proteins Cyclic AMP-Dependent Protein Kinases/metabolism Lipase Lipolysis/physiology Mice Mice, Transgenic Microscopy, Fluorescence Perilipin-1 Phosphoproteins/genetics,metabolism Sterol Esterase/metabolism
Chemicals
Carrier Proteins Perilipin-1 Phosphoproteins Cyclic AMP-Dependent Protein Kinases Carboxylic Ester Hydrolases Sterol Esterase Lipase PNPLA2 protein, mouse
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miyoshi Hideaki
Jean Mayer United States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, Massachusetts 02111, USA.
Perfield James W
Obin Martin S
Greenberg Andrew S
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Article Info
Journal
Journal of cellular biochemistry
Abbr.
J Cell Biochem
ISSN
1097-4644
DOI
10.1002/jcb.21964
Published
2008-12-15
Pages
1430-6
Language
English
Region
United States
NLM ID
8205768
PMCID
PMC2593643
Subset
IM
Grants
NIDDK NIH HHS · R01 DK050647-08 · United States
NIDDK NIH HHS · P30 DK034928 · United States
NIDDK NIH HHS · P30 DK-34928 · United States
NIA NIH HHS · R03 AG024635 · United States
NIDDK NIH HHS · DK-50647 · United States
NIDDK NIH HHS · R01 DK050647 · United States
NIA NIH HHS · AG024635 · United States
NINDS NIH HHS · P30 NS047243 · United States
External Links
PubMed source DOI: 10.1002/jcb.21964 PMC Full Text View journal details
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