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PMID: 18981049 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Proteome-wide identification of poly(ADP-ribose) binding proteins and poly(ADP-ribose)-associated protein complexes.

Nucleic acids research ·Vol. 36 ·No. 22 ·2008-12-00 ·Pages 6959-76

Gagné JP, Isabelle M, Lo KS, Bourassa S, Hendzel MJ, Dawson VL, Dawson TM, Poirier GG

Abstract

Poly(ADP-ribose) (pADPr) is a polymer assembled from the enzymatic polymerization of the ADP-ribosyl moiety of NAD by poly(ADP-ribose) polymerases (PARPs). The dynamic turnover of pADPr within the cell is essential for a number of cellular processes including progression through the cell cycle, DNA repair and the maintenance of genomic integrity, and apoptosis. In spite of the considerable advances in the knowledge of the physiological conditions modulated by poly(ADP-ribosyl)ation reactions, and notwithstanding the fact that pADPr can play a role of mediator in a wide spectrum of biological processes, few pADPr binding proteins have been identified so far. In this study, refined in silico prediction of pADPr binding proteins and large-scale mass spectrometry-based proteome analysis of pADPr binding proteins were used to establish a comprehensive repertoire of pADPr-associated proteins. Visualization and modeling of these pADPr-associated proteins in networks not only reflect the widespread involvement of poly(ADP-ribosyl)ation in several pathways but also identify protein targets that could shed new light on the regulatory functions of pADPr in normal physiological conditions as well as after exposure to genotoxic stimuli.

MeSH Terms
Amino Acid Motifs Carrier Proteins/metabolism Cell Line, Tumor Chromatography, Liquid DNA Damage Electrophoresis, Gel, Two-Dimensional HeLa Cells Humans Immunoprecipitation Models, Biological Poly Adenosine Diphosphate Ribose/chemistry,metabolism Proteins/chemistry,metabolism Proteome/metabolism Tandem Mass Spectrometry
Chemicals
Carrier Proteins Proteins Proteome poly(ADP)-ribosylated proteins Poly Adenosine Diphosphate Ribose
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gagné Jean-Philippe
Laval University Medical Research Center, CHUQ, Faculty of Medicine, Laval University, Québec, Canada.
Isabelle Maxim
Lo Ken Sin
Bourassa Sylvie
Hendzel Michael J
Dawson Valina L
Dawson Ted M
Poirier Guy G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkn771
Published
2008-12-00
Epub
2008-00-03
Pages
6959-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC2602769
Subset
IM
Grants
PHS HHS · NINDS 039148 · United States
External Links
PubMed source DOI: 10.1093/nar/gkn771 PMC Full Text View journal details
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